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Direct detection of a sulfonate ester genotoxic impurity by atmospheric-pressure thermal desorption−extractive electrospray−mass spectrometry
journal contribution
posted on 2013-07-10, 14:10 authored by Neil A. Devenport, Laura C. Sealey, Faisal H. Alruways, Daniel J. Weston, Jim ReynoldsJim Reynolds, Colin CreaserA direct, ambient ionization method has been developed using atmospheric pressure thermal desorption−extractive electrospray−mass spectrometry (AP/TD-EESI-MS) for the detection of the genotoxic impurity (GTI) methyl p-toluenesulfonate (MTS) in a surrogate pharmaceutical matrix. A custom-made thermal desorption probe was used to the desorb and vaporize MTS from the solid state, by rapid heating to 200 °C then cooling to ambient temperature, with a cycle time of 6 min. The detection of MTS using EESI with a sodium acetate doped solvent to generate the [MTS+Na]+ adduct ion provided a significant sensitivity enhancement relative to the [M+H]+ ion generated using a 0.1% formic acid solvent modifier. The MTS detection limit is over an order of magnitude below the longterm daily threshold of toxicological concern (TTC) of 1.5 μg/g and the potential for quantitative analysis has been determined using starch as a surrogate active pharmaceutical ingredient (API).
Funding
This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) and AstraZeneca [grant number BB/G017557/1].
History
School
- Science
Department
- Chemistry
Citation
DEVENPORT, N.A. ... et al., 2013. Direct detection of a sulfonate ester genotoxic impurity by atmospheric-pressure thermal desorption−extractive electrospray−mass spectrometry. Analytical Chemistry, 85 (13), pp. 6224 - 6227.Publisher
© American Chemical SocietyVersion
- NA (Not Applicable or Unknown)
Publication date
2013Notes
This article is closed access.eISSN
1520-6882Publisher version
Language
- en