Thesis-2008-Young.pdf (16.77 MB)
Nebulized flame ionization detection in high performance liquid chromatography
thesis
posted on 2014-07-07, 12:47 authored by Erepamowei YoungA nebulized flame ionization detector interfaced with LC was examined and
found to be more versatile in applications than common LC detectors, such
as UV, RID, ELSD and CAD. The technique can be used for both volatile
and non-volatile analytes. It is compatible with gradient elution and can be
used for the analysis of non chromophore-possessing analytes. The
calibration plots of non-volatile analytes were linear in contrast to other
aerosol-based detectors, such as ELSD and CAD.
The technique was examined in three consecutive stages; optimization of the
FID, testing the response patterns of analytes (volatile and non-volatile) and
applications to the analysis of compounds of diverse functional groups.
The optimum conditions for the operation of the FID were: hydrogen, 157
ml/min; nitrogen, 250 ml/min; air, 654 ml/min; spray chamber internal
diameter, 40 mm, collector internal diameter, 4mm and eluent (water), 1
ml/min.
The calibration plots of all volatile analytes were linear while those of the
non-volatile analytes were linear only when anions (in the form of sulfuric
acid, nitric acid, hydrochloric acid, orthophosphoric acid, sodium sulphate
and ammonium sulphate) were added to the eluent
The separations of diverse analytes (alcohols, aldehydes, ketones, amines,
amino acids, carboxylic acids and sugars) gave detection limits in the low μg
range.
History
School
- Science
Department
- Chemistry
Publisher
© Erepamowei YoungPublication date
2008Notes
A Doctoral Thesis. Submitted in partial fulfillment of the requirements for the award of Doctor of Philosophy of Loughborough University.EThOS Persistent ID
uk.bl.ethos.603096Language
- en