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Anharmonic surface interactions for biomolecular screening and characterization

journal contribution
posted on 2014-09-09, 14:26 authored by Sourav GhoshSourav Ghosh, Victor P. Ostanin, Ashwin A. Seshia
The acoustic response of conventional mechanical oscillators, such as a piezoelectric crystal, is predominantly harmonic at modest amplitudes. However, here, we observe from the electrical response that significant motional anharmonicity is introduced in the presence of attached analyte. Experiments were conducted with streptavidin-coated polystyrene microbeads of various sizes attached to a quartz crystal resonator via specific and nonspecific molecular tethers in liquid. Quantitative analysis reveals that the deviation of odd Fourier harmonics of the response caused by introduction of microbeads as a function of oscillation amplitude presents a unique signature of the molecular tether. Hence, the described anharmonic detection technique (ADT) based on this function allows screening of biomolecules and provides an additional level of selectivity in receptor-based detection that is often associated with nonspecific interactions. We also propose methods to extract mechanical force-extension characteristics of the molecular tether and activation energy using this technique. © 2010 American Chemical Society.

Funding

This project was funded in part by the Engineering and Physical Sciences Research Council and the Cambridge Commonwealth Trust.

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

Analytical Chemistry

Volume

83

Issue

2

Pages

549 - 554

Citation

GHOSH, S.K., OSTANIN, V.P. and SESHIA, A.A., 2011. Anharmonic surface interactions for biomolecular screening and characterization. Analytical Chemistry, 83 (2), pp. 549 - 554

Publisher

© American Chemical Society

Version

  • AM (Accepted Manuscript)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2011

Notes

This article is closed access.

ISSN

0003-2700

Language

  • en

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