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Ratiometric detection of adenosine triphosphate (ATP) in water and real-time monitoring of apyrase activity with a tripodal zinc complex

journal contribution
posted on 2015-09-15, 15:16 authored by Stephen ButlerStephen Butler
Two tripodal fluorescent probes Zn⋅L1,2 have been synthesised, and their anion-binding capabilities were examined by using fluorescence spectroscopy. Probe Zn⋅L1 allows the selective and ratiometric detection of adenosine triphosphate (ATP) at physiological pH, even in the presence of several competing anions, such as ADP, phosphate and bicarbonate. The probe was applied to the real-time monitoring of the apyrase-catalysed hydrolysis of ATP, in a medium that mimics an extracellular fluid.

Funding

S.J.B. wishes to thank the Ramsay Memorial Fellowship Trust and Durham University for support and funding.

History

School

  • Science

Department

  • Chemistry

Published in

CHEMISTRY-A EUROPEAN JOURNAL

Volume

20

Issue

48

Pages

15768 - 15774 (7)

Citation

BUTLER, S.J., 2014. Ratiometric detection of adenosine triphosphate (ATP) in water and real-time monitoring of apyrase activity with a tripodal zinc complex. Chemistry - A European Journal, 20 (48), pp. 15768 - 15774.

Publisher

© Wiley-VCH Verlag GmbH & Co.

Version

  • VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2014

Notes

This article is closed access.

ISSN

0947-6539

Language

  • en

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