acs%2Eanalchem%2E5b04663.pdf (987.96 kB)
Detection of matrilysin (MMP-7) activity using polypeptide functionalized reduced graphene oxide field-effect transistor sensor.
journal contribution
posted on 2016-03-30, 10:01 authored by Hu Chen, Peng Chen, Jingfeng Huang, Robert Selegard, Mark PlattMark Platt, Alagappan Palaniappan, Daniel Aili, Alfred I.Y. Tok, Bo LiedbergA novel approach for rapid and sensitive detection of matrilysin (MMP-7, a biomarker involved in the degradation of vari-ous macromolecules) based on polypeptide (JR2EC) functionalized reduced graphene oxide (rGO) field effect transistor (FET) is reported. MMP-7 specifically digests negatively charged JR2EC immobilized on rGO, thereby modulating the con-ductance of rGO-FET. The proposed assay enabled detection of MMP-7 at clinically relevant concentrations with a limit of detection (LOD) of 10 ng/mL (400 pM), attributed to the significant reduction of the net charge of JR2EC upon digestion by MMP-7. Quantitative detection of MMP-7 in human plasma was further demonstrated with a LOD of 40 ng/mL, illustrating the potential for the proposed methodology for tumor detection and carcinoma diagnostic (e.g. lung cancer and salivary gland cancer). Additionally, excellent specificity of the proposed assay was demonstrated using matrix metallopeptidase 1 (MMP-1), a protease of the same family. With appropriate selection and modification of polypeptides, the proposed assay could be extended for detections of other enzymes with polypeptide digestion capability.
Funding
This research was funded by the Institute for Sports Research, Nanyang Technological University
History
School
- Science
Department
- Chemistry
Published in
Analytical chemistryCitation
CHEN, H. ... et al, 2016. Detection of matrilysin (MMP-7) activity using polypeptide functionalized reduced graphene oxide field-effect transistor sensor. Analytical Chemistry, 88 (6), pp. 2994-2998.Publisher
© American Chemical SocietyVersion
- AM (Accepted Manuscript)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2016-02-18Notes
This article was published in Analytical Chemistry [© American Chemical Society] and the definitive version is available at: http://dx.doi.org/10.1021/acs.analchem.5b04663ISSN
0003-2700eISSN
1520-6882Publisher version
Language
- en