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Automated adherent human cell culture (mesenchymal stem cells)
journal contribution
posted on 2017-08-21, 10:08 authored by Rob ThomasRob Thomas, Elizabeth RatcliffeElizabeth RatcliffeHuman cell culture processes developed at research laboratory scale need to be translated to large-scale production processes to achieve commercial application to a large market. To allow this transition of scale with consistent process performance and control of costs, it will be necessary to reduce manual processing and increase automation. There are a number of commercially available platforms that will reduce manual process intervention and improve process control for different culture formats. However, in many human cell-based applications, there is currently a need to remain close to the development format, usually adherent culture on cell culture plastic or matrix-coated wells or flasks due to deterioration of cell quality in other environments, such as suspension. This chapter presents an example method for adherent automated human stem cell culture using a specific automated flask handling platform, the CompacT SelecT.
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Methods in Molecular BiologyVolume
806Pages
393 - 406Citation
THOMAS, R. and RATCLIFFE, E., 2012. Automated adherent human cell culture (mesenchymal stem cells). IN: Mitry, R. and Hughes, R. (eds.). Human Cell Culture Protocols. (Methods in Molecular Biology ; vol. 806.) London: Springer, pp.393-406.Publisher
© SpringerVersion
- NA (Not Applicable or Unknown)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2012ISBN
9781617793660;9781617793677ISSN
1064-3745eISSN
1940-6029Publisher version
Book series
Methods in Molecular Biology;806Language
- en