File(s) under permanent embargo
Reason: This item is currently closed access.
Laser ablation-inductively coupled plasma mass spectrometry: An emerging technology for detecting rare cells in tissue sections
journal contribution
posted on 2017-09-28, 10:51 authored by Amy ManaghAmy Managh, Robert W. Hutchinson, Paloma Riquelme, Christiane Broichhausen, Anja K. Wege, Uwe Ritter, Norbert Ahrens, Gudrun E. Koehl, Lisa Walter, Christian Florian, Hans J. Schlitt, Helen Reid, Edward K. Geissler, Barry Sharp, James A. Hutchinson© 2014 by The American Association of Immunologists, Inc. Administering immunoregulatory cells to patients as medicinal agents is a potentially revolutionary approach to the treatment of immunologically mediated diseases. Presently, there are no satisfactory, clinically applicable methods of tracking human cells in patients with adequate spatial resolution and target cell specificity over a sufficient period of time. Laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS) represents a potential solution to the problem of detecting very rare cells in tissues. In this article, this exquisitely sensitive technique is applied to the tracking of gold-labeled human regulatory macrophages (Mregs) in immunodeficient mice. Optimal conditions for labeling Mregs with 50-nm gold particles were investigated by exposing Mregs in culture to variable concentrations of label: Mregs incubated with 3.5 × 10 9 particles/ml for 1 h incorporated an average of 3.39 × 10 8 Au atoms/cell without loss of cell viability. Analysis of single, gold-labeled Mregs by LA-ICP-MS registered an average of 1.9 × 10 5 counts/cell. Under these conditions, 100% labeling efficiency was achieved, and label was retained by Mregs for ≥36 h. Gold-labeled Mregs adhered to glass surfaces; after 24 h of culture, it was possible to colabel these cells with humanspecific 154 Sm-tagged anti-HLA-DR or 174 Yb-tagged anti-CD45 mAbs. Following injection into immunodeficient mice, signals from gold-labeled human Mregs could be detected in mouse lung, liver, and spleen for at least 7 d by solution-based inductively coupled plasma mass spectrometry and LA-ICP-MS. These promising results indicate that LA-ICP-MS tissue imaging has great potential as an analytical technique in immunology. Copyright
History
School
- Science
Department
- Chemistry
Published in
Journal of ImmunologyVolume
193Issue
5Pages
2600 - 2608Citation
MANAGH, A.J., 2014. Laser ablation-inductively coupled plasma mass spectrometry: An emerging technology for detecting rare cells in tissue sections. Journal of Immunology, 193(5), pp. 2600 - 2608.Publisher
American Association of ImmunologistsVersion
- AM (Accepted Manuscript)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2014-09-01Notes
This paper is in closed access.ISSN
0022-1767eISSN
1550-6606Publisher version
Language
- en