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In vitro investigation of cellular effects of magnesium and magnesium-calcium alloy corrosion products on skeletal muscle regeneration
journal contribution
posted on 2019-02-01, 10:05 authored by Diana Maradze, Andrew CapelAndrew Capel, Neil MartinNeil Martin, Mark LewisMark Lewis, Yufeng Zheng, Yang LiuYang LiuBiodegradable magnesium (Mg) has garnered attention for its use in orthopaedic
implants due to mechanical properties that closely match to those of bone.
Studies have been undertaken to understand the corrosion behaviour of these
materials and their effects on bone forming cells. However, there is lack of
research on how the corrosion of these biomaterials affect surrounding tissues
such as skeletal muscle. Mg plays an important role in the structural and
functional properties of skeletal muscle. It is therefore important to investigate
the response of skeletal muscle cells to both soluble (Mg ions) and insoluble
(corrosion granules) corrosion products. Through in vitro studies it is possible to
observe the effects of corrosion products on myotube formation by the fusion of
single muscle precursor cells known as myoblasts. To achieve this goal, it is first
important to determine if these corrosion products are toxic to myotubes. Here it
was noted that although there was a slight decrement in cellular viability after
initial exposure, this soon recovered to control levels. A high Ca/Mg ratio
resulted in the formation of large myotubes and a low Ca/Mg ratio negatively
affected myotube maturation. Mg2+ and Ca2+ ions are important in the process of
myogenesis, the concentration of these ions and the ratio of the ions to each other
played a significant role in myotube cellular activity. The outcomes of this study could pave the way to a bio-informed and integrated approach to the design and
engineering of Mg-based orthopaedic implants.
Funding
DM’s PhD studentship was supported by The EPSRC (EP/F500491/1) Centre of Doctor Training in Regenerative Medicine and SkelGen under Marie Sklodowska-Curie Research and Innovation Staff Exchange programme (FP7-PEOPLE-2012-IRSES, 318553).
History
School
- Mechanical, Electrical and Manufacturing Engineering
- Sport, Exercise and Health Sciences
Published in
Journal of Materials Science and TechnologyVolume
35Issue
11Pages
2503 - 2512Citation
MARADZE, D. ... et al, 2019. In vitro investigation of cellular effects of magnesium and magnesium-calcium alloy corrosion products on skeletal muscle regeneration. Journal of Materials Science and Technology, 35 (11), pp.2503-2512.Publisher
© Elsevier on behalf of The editorial office of Journal of Materials Science & TechnologyVersion
- AM (Accepted Manuscript)
Publisher statement
This paper was accepted for publication in the journal Journal of Materials Science and Technology and the definitive published version is available at https://doi.org/10.1016/j.jmst.2019.01.020.Acceptance date
2019-01-11Publication date
2019-07-10ISSN
1005-0302Publisher version
Language
- en