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Direct manipulation of liquid ordered lipid membrane domains using optical traps

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posted on 2019-02-11, 14:33 authored by Mark S. Friddin, Guido Bolognesi, Ali Salehi-Reyhani, Oscar Ces, Yuval Elani
Multicomponent lipid bilayers can give rise to coexisting liquid domains that are thought to influence a host of cellular activities. There currently exists no method to directly manipulate such domains, hampering our understanding of their significance. Here we report a system that allows individual liquid ordered domains that exist in a liquid disordered matrix to be directly manipulated using optical tweezers. This allows us to drag domains across the membrane surface of giant vesicles that are adhered to a glass surface, enabling domain location to be defined with spatiotemporal control. We can also use the laser to select individual vesicles in a population to undergo mixing/demixing by locally heating the membrane through the miscibility transition, demonstrating a further layer of control. This technology has potential as a tool to shed light on domain biophysics, on their role in biology, and in sculpting membrane assemblies with user-defined membrane patterning.

Funding

This work was supported by the EPSRC via grant EP/J017566/1 and by EPSRC Fellowship EP/N016998/1 awarded to Y.E.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Communications Chemistry

Volume

2

Issue

1

Citation

FRIDDIN, M.S. ... et al., 2019. Direct manipulation of liquid ordered lipid membrane domains using optical traps. Communications Chemistry, 2: 6.

Publisher

© the Authors. Published by Springer Nature

Version

  • VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/ by/4.0/

Acceptance date

2018-11-16

Publication date

2019-01-29

Notes

This is an Open Access Article. It is published bySpringer under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/

eISSN

2399-3669

Language

  • en

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