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Induction studies with Escherichia coli expressing recombinant interleukin-13 using multi-parameter flow cytometry
journal contribution
posted on 2010-01-08, 12:44 authored by Jennifer O. Shitu, John M. Woodley, Richard Wnek, Michel J. Chartrain, Christopher HewittThe expression of interleukin -13 following induction with IPTG in E. coli results in metabolic changes as indicated by multi-parameter flow cytometry and traditional methods of fermentation profiling (O2 uptake rate, CO2 evolution rate and optical density measurements). Induction early in the rapid growth phase was optimal although this led to lower overall biomass concentrations and lower maximum specific growth rates. In contrast, induction in the mid-rapid growth phase was the most detrimental to cell quality as measured by cytoplamsic membrane depolarisation.
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Citation
SHITU, J.O. ... et al, 2009. Induction studies with Escherichia coli expressing recombinant interleukin-13 using multi-parameter flow cytometry. Biotechnology Letters, 31 (4), pp. 577-584.Publisher
© Springer VerlagVersion
- AM (Accepted Manuscript)
Publication date
2009Notes
This article was published in the journal, Biotechnology Letters [© Springer Verlag] and the original publication is available at www.springerlink.comISSN
0141-5492;1573-6776Language
- en