Thesis-2014-Managh.pdf (12.98 MB)
Single-cell tracking of therapeutic cells using Laser Ablation–Inductively Coupled Plasma–Mass Spectrometry
thesis
posted on 2015-02-12, 16:18 authored by Amy J. ManaghCellular therapy is emerging as a clinically viable strategy in the field of solid organ
transplantation, where it is expected to reduce the dependency on conventional
immunosuppression. This has produced a demand for highly sensitive methods to
monitor the persistence and tissue distribution of administered cells in vivo. However,
tracking cells presents significant challenges. In many cases transplanted cells are
autologous with the immune system of the transplant recipient, and hence are invisible
to typical methods of detection. To enable their differentiation, the cells must be
labelled with a suitable, non-toxic and long lifetime label, prior to their administration to
patients. In addition, administered cells represent only a small fraction of the
recipient’s endogenous cells, which necessitates the use of an extremely sensitive
detection method. Laser ablation – inductively coupled plasma – mass spectrometry
(LA-ICP-MS) is an exquisitely sensitive analytical technique, capable of imaging trace
elements in complex samples, at high spatial resolution. [Continues.]
Funding
European Commission 7th Framework Program
History
School
- Science
Department
- Chemistry
Publisher
© Amy Joan ManaghPublisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2014Notes
A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy of Loughborough University.Language
- en