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An overview of current techniques for ocular toxicity testing
journal contribution
posted on 2016-11-18, 15:06 authored by Sammy WilsonSammy Wilson, Mark Ahearne, Andrew HopkinsonGiven the hazardous nature of many materials and substances, ocular toxicity testing is required to evaluate the dangers associated with these substances after their exposure to the eye. Historically, animal tests such as the Draize test were exclusively used to determine the level of ocular toxicity by applying a test substance to a live rabbit’s eye and evaluating the biological response. In recent years, legislation in many developed countries has been introduced to try to reduce animal testing and promote alternative techniques. These techniques include ex vivo tests on deceased animal tissue, computational models that use algorithms to apply existing data to new chemicals and in vitro assays based on two dimensional (2D) and three dimensional (3D) cell culture models. Here we provide a comprehensive overview of the latest advances in ocular toxicity testing techniques, and discuss the regulatory framework used to evaluate their suitability.
Funding
Funding from EPSRC Engineering, Tissue Engineering and Regenerative Medicine Fellowship (E-TERM, Grant number: EP/ 1017801/1) and the University of Nottingham HERMES Fellowship (Grant number: 13b/I9)
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
ToxicologyVolume
327Pages
32 - 46Citation
WILSON, S.L., AHEARNE, M. and HOPKINSON, A., 2014. An overview of current techniques for ocular toxicity testing. Toxicology, 327, pp. 32-46.Publisher
Elsevier © The AuthorsVersion
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/Publication date
2014Notes
This is an Open Access article. It is published by Elsevier under the Creative Commons Attribution 3.0 Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/ISSN
0300-483XPublisher version
Language
- en