File(s) under permanent embargo
Reason: This item is currently closed access.
The Development of a ɛ-Polycaprolactone Scaffold for Central Nervous System Repair
journal contribution
posted on 2017-05-12, 09:48 authored by Peter S. Donoghue, Rebecca Lamond, Stephanie D. Boomkamp, Tao SunTao Sun, Nikolaj Gadegaard, Mathis O. Riehle, Susan C. BarnettPotential treatment strategies for the repair of spinal cord injury (SCI)
currently favor a combinatorial approach incorporating several factors,
including exogenous cell transplantation and biocompatible scaffolds.
The use of scaffolds for bridging the gap at the injury site is very
appealing although there has been little investigation into the central
nervous system neural cell interaction and survival on such scaffolds
before implantation. Previously, we demonstrated that aligned
microgrooves 12.5-25 μm wide on ε-polycaprolactone (PCL) promoted
aligned neurite orientation and supported myelination. In this study, we
identify the appropriate substrate and its topographical features required
for the design of a three-dimensional scaffold intended for
transplantation in SCI. Using an established myelinating culture system
of dissociated spinal cord cells, recapitulating many of the features of
the intact spinal cord, we demonstrate that astrocytes plated on the
topography secrete soluble factors(s) that delay oligodendrocyte
differentiation, but do not prevent myelination. However, as myelination
does occur after a further 10-12 days in culture, this does not prevent
the use of PCL as a scaffold material as part of a combined strategy for
the repair of SCI.
Funding
The work described in the article was funded by the Biotechnology and Biological Sciences Research Council PCL SCAFFOLD FOR CNS REPAIR 505 (BB/G004706/1) (BBSRC, PD, ST); Lord Kelvin and Adam Smith PhD studentship (RL) and NC3Rs (SDB).
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Tissue Engineering Part AVolume
19Issue
3-4Pages
497 - 507Citation
DONOGHUE, P.S. ... et al, 2013. The Development of a ɛ-Polycaprolactone Scaffold for Central Nervous System Repair. Tissue Engineering Part A, 19 (3-4), pp.497-507Publisher
© Mary Ann Liebert, Inc. publishersVersion
- NA (Not Applicable or Unknown)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2013Notes
This paper is closed access.ISSN
1937-3341eISSN
1937-335XPublisher version
Language
- en