Pulsed-source luminescence measurements using a computer-controlled spectrometer

Traditionally fluorescence and phosphorescence measurements are made on fluorescence spectrometers using a DC source such as a 150 Watt xenon lamp. Differentiation between the fast decaying fluorescence signal and the relatively long lived phosphorescence is temporally obtained using a mechanical phosphoroscope. By periodically interrupting the exciting light measurements are made at the instant of excitation (fluorescence) or during the periods of darkness (phosphorescence). [Continues.]