Dual Aptamer Paper_Final.pdf (789.79 kB)
The design and characterization of multifunctional aptamer nanopore sensors
journal contribution
posted on 2018-05-29, 12:16 authored by Laura J. Mayne, Chih-Yuan Lin, Steven ChristieSteven Christie, Zuzanna S. Siwy, Mark PlattMark PlattAptamer-modified nanomaterials provide a
simple, yet powerful sensing platform when combined with resistive pulse sensing technologies. Aptamers adopt a more stable tertiary structure in the presence of a target analyte, which results in a change in charge density and velocity of the carrier particle. In practice the tertiary structure is specific for each aptamer and target, and the strength of the signal varies with different applications and experimental conditions. Resistive pulse sensors (RPS) have single
particle resolution, allowing for the detailed characterization of the sample. Measuring the velocity of aptamer-modified nanomaterials as they traverse the RPS provides information on their charge state and densities. To help understand how the aptamer structure and charge density effects the sensitivity of aptamer-RPS assays, here we study two metal binding
aptamers. This creates a sensor for mercury and lead ions that is capable of being run in a range of electrolyte concentrations, equivalent to river to seawater conditions. The observed results are in excellent agreement with our
proposed model. Building on this we combine two aptamers together in an attempt to form a dual sensing strand of DNA for the simultaneous detection of two metal ions. We show experimental and theoretical responses for the aptamer which creates layers of differing charge densities around the nanomaterial. The density and diameter of these zones effects both the viability and sensitivity of the assay. While this approach allows the interrogation of the DNA structure, the data also highlight the limitations and considerations for future assays.
Funding
Z.S. and C.Y.L. acknowledge Horizon Development, LLC for financial support.
History
School
- Science
Department
- Chemistry
Published in
ACS NanoVolume
12Issue
5Pages
4844 - 4852Citation
MAYNE, L.J. ... et al, 2018. The design and characterization of multifunctional aptamer nanopore sensors. ACS Nano, 12 (5), pp.4844-4852.Publisher
© American Chemical SocietyVersion
- AM (Accepted Manuscript)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Acceptance date
2018-05-01Publication date
2018-05-02Notes
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acsnano.8b01583ISSN
1936-0851eISSN
1936-086XPublisher version
Language
- en