STEROIDSrevised.pdf (248.48 kB)
High temperature liquid chromatography of steroids on a bonded hybrid column
journal contribution
posted on 2009-01-15, 16:34 authored by Lateefa A. Al-Khateeb, Roger SmithA hybrid stationary phase, XTerra MS C18, has been evaluated for the high
temperature reversed phase liquid chromatography of selected hydrophobic steroids. The
effects on the retention and efficiency at temperatures up to 130 °C and eluent
compositions from methanol-water mixtures to superheated water were studied. The
thermodynamic data of the separations were determined. It was shown that increasing the
temperature enabled the percentage of methanol to be reduced. High mobile phase flow
rates could be used but for these non-polar analytes the retention times with superheated
water as the eluent were still high.
History
School
- Science
Department
- Chemistry
Citation
AL-KHATEEB, L.A. and SMITH, R.M., 2009. High temperature liquid chromatography of steroids on a bonded hybrid column. Analytical and Bioanalytical Chemistry, 394 (5), pp. 1255–1260Publisher
© Springer VerlagVersion
- AM (Accepted Manuscript)
Publication date
2009Notes
This article was acepted for publication in the journal, Analytical and Bioanalytical Chemistry [© Springer-Verlag] and will be available at: http://www.springer.com/chemistry/analytical/journal/216ISSN
1618-2642;1618-2650Language
- en