AN-ART-03-2012-035432.pdf (1014.77 kB)
Sampling and characterisation of volatile organic compound profiles in human saliva using a polydimethylsiloxane coupon placed within the oral cavity
journal contribution
posted on 2012-06-13, 10:47 authored by Helen J. Martin, Svetlana Riazanskaia, Paul ThomasEvaluation of published methods reveals that existing methods for saliva sampling do not
address the physical chemical attributes of volatile organic compounds (VOC). This study
describes and presents evidence for adopting in-situ sampling of salivary VOC directly from
the oral cavity using a polydimethylsiloxane (PDMS) based sampler. In-vitro studies indicated
that the vapour pressure of analytes was a factor in both the recovery of analytes, and in the
precision of the recovery. The highest recoveries were observed for VOC with the lowest
vapour pressures, for example 5-nonanol (vapour pressure (Pv) = 14 PA) recoveries were
approximately 20-times greater than those observed for octane (Pv = 1726 PA). Similarly,
relative standard deviations reduced with vapour pressure, with the RSD for 5-nonanol
responses observed to be 2.7 % to compared to RSD = 26 % for octane. Evaluation of VOC
recovered from 6 in-vivo samples indicated that VOC concentrations in saliva may follow lognormal
distributions; log-normal RSDs falling between 4.4% to 18.2% across the range of
volatilities encountered. Increasing sampling time from 1 to 30 minutes indicated that the
recovery of VOC into the sampler was effected by interaction between different physical
chemical properties and biogenic flux. A sampling time of 10 min was found to offer an
acceptable compromise that enabled a representative sample to be acquired for the widest
range of observed VOC behaviours with the sampler. The potential to ‘tune’ the sampling
protocol for targeted analysis based on these factors was also noted.
Comparison with passive drool saliva collection revealed up to 105 enhancment with
reduced variability compared to drooled samples. This approach to in-situ saliva sampling
appears to have significant analytical utility for studying volatile signatures in humans.
History
School
- Science
Department
- Chemistry
Citation
MARTIN, H.J., RIAZANSKAIA, S. and THOMAS, C.L.P., 2012. Sampling and characterisation of volatile organic compound profiles in human saliva using a polydimethylsiloxane coupon placed within the oral cavity. Analyst, 137, pp. 3627–3634.Publisher
RSC Publishing (© H.J. Martin, S. Riazanskaia and C.L.P. Thomas)Version
- AM (Accepted Manuscript)
Publication date
2012Notes
This article was published in the journal, Analyst [RSC Publishing] and the definitive version is available at: http://dx.doi.org/10.1039/C2AN35432BISSN
0003-2654eISSN
1364-5528Publisher version
Language
- en