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|Title: ||Near infrared fluorescence spectroscopy|
|Authors: ||Summerfield, Stephen|
|Issue Date: ||1993|
|Publisher: ||© Stephen Summerfield|
|Abstract: ||Fluorimetry in the very near infrared region ca. 600-1000nm is a new
approach to photochemical analysis. The advantages include greatly reduced
background fluorescence signals from important sample matrices (such as blood
serum), reduced scattering, and reduced probability of sample decomposition. Also,
the availability of low cost, efficient, stable and robust optical components (e.g.
laser diodes and light emitting diodes), solid state detectors (e.g. single silicon
photodiodes and diode arrays) and fibre optics, allows the construction of an
inexpensive fluorimeter. In the near infrared region, there are some very bright
fluorophores that can be adapted for use as fluorescent probes, labels for
immunoassay, and as ion-pair agents.
The advantageous performance of most types of fluorimetric analysis now
undertaken In the ultraviolet and visible region of the spectrum may therefore be
extended into the longer wavelength region. Excellent limits of detection are
attainable, and some near infrared fluorophores show invaluable fluorescence probe
properties, such as Nile Red.
The most useful of the dye groups investigated were the phenoxazines and
thiazines. Reactive derivatives of these dyes show great potential as fluorescent
labels for Immunoassay. These dyes have also been used as probes due to their
solvatochromism and sensitivity to pH.|
|Description: ||Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy of Loughborough University.|
|Appears in Collections:||PhD Theses (Chemistry)|
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