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Title: Large-scale expansion and exploitation of pluripotent stem cells for regenerative medicine purposes: beyond the T flask
Authors: Want, Andrew
Nienow, Alvin W.
Hewitt, Christopher J.
Coopman, Karen
Keywords: Automation
Large scale
Pluripotent stem cells
Suspension culture
Issue Date: 2012
Publisher: © Future Medicine Ltd
Citation: WANT, A. ... et al., 2012. Large-scale expansion and exploitation of pluripotent stem cells for regenerative medicine purposes: beyond the T flask. Regenerative Medicine, 7 (1), pp.71-84.
Abstract: Human pluripotent stem cells will likely be a significant part of the regenerative medicine-driven healthcare revolution. In order to realize this potential, culture processes must be standardized, scalable and able to produce clinically relevant cell numbers, whilst maintaining critical biological functionality. This review comprises a broad overview of important bioprocess considerations, referencing the development of biopharmaceutical processes in an effort to learn from current best practice in the field. Particular focus is given to the recent efforts to grow human pluripotent stem cells in microcarrier or aggregate suspension culture, which would allow geometric expansion of productive capacity were it to be fully realized. The potential of these approaches is compared with automation of traditional T-flask culture, which may provide a cost-effective platform for low-dose, low-incidence conditions or autologous therapies. This represents the first step in defining the full extent of the challenges facing bioprocess engineers in the exploitation of large-scale human pluripotent stem cell manufacture.
Description: This is the submitted for publication version of an article which was published in the journal, Regenerative Medicine [© Future Medicine Ltd] and the definitive version is available at: http://dx.doi.org/10.2217/rme.11.101
Version: Submitted for publication
DOI: 10.2217/RME.11.101
URI: https://dspace.lboro.ac.uk/2134/11041
Publisher Link: http://dx.doi.org/10.2217/RME.11.101
ISSN: 1746-0751
Appears in Collections:Published Articles (Chemical Engineering)

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