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Title: Caffeine ingestion and antigen-stimulated human lymphocyte activation after prolonged cycling
Authors: Fletcher, Deborah K.
Bishop, Nicolette
Keywords: Methylxanthine
Immune cells
Exercise
Issue Date: 2012
Publisher: © John Wiley and Sons
Citation: FLETCHER, D.K. and BISHOP, N., 2012. Caffeine ingestion and antigen-stimulated human lymphocyte activation after prolonged cycling. Scandinavian Journal of Medicine and Science in Sports, 22 (2), pp. 249 - 258
Abstract: This study investigated the effect of caffeine ingestion on antigen-stimulated T- (CD4+ and CD8+) and natural killer (NK)- (CD3−CD56+) cell activation after prolonged, strenuous cycling. In a randomized cross-over design, nine male endurance cyclists (age: 22 ± 3 years, V̇O2peak: 62 ± 4 mL/kg/min, mean ± SD) cycled for 90 min at 70% V̇O2peak 60 min after ingesting 6 mg/kg body mass of caffeine (CAF) or placebo (PLA). Venous blood samples were obtained before supplementation, pre-exercise, immediately post-exercise and 1 h post-exercise. Whole blood was stimulated with Pediacel (five in one) vaccine. At 1 h post-exercise the number of antigen-stimulated CD4+ cells expressing CD69 decreased on CAF compared with PLA [15 (17) × 106 vs 23 (22) × 106 cells/L, P<0.05]. In addition, the geometric mean fluorescence intensity (GMFI) of CD69 expression on antigen-stimulated CD8+ cells decreased on CAF compared with PLA 1 h post-exercise [78 (10)% vs 102 (24)%, P<0.05]. At the same time-point GMFI of CD69 expression on antigen-stimulated CD3−CD56+ cells was increased on CAF compared with PLA [103 (9)% vs 87 (8)%, P<0.05]. These findings suggest that caffeine reduces antigen-stimulated CD69 expression on T cells while at the same time increases NK-cell activation 1 h after intensive cycling.
Description: This article is closed access, it was published in Scandinavian Journal of Medicine and Science in Sports [© John Wiley and sons]. The definitive version is available at: http://dx.doi.org/10.1111/j.1600-0838.2010.01223.x
Version: Accepted for publication
DOI: 10.1111/j.1600-0838.2010.01223.x
URI: https://dspace.lboro.ac.uk/2134/11182
Publisher Link: http://dx.doi.org/10.1111/j.1600-0838.2010.01223.x
ISSN: 0905-7188
Appears in Collections:Closed Access (Sport, Exercise and Health Sciences)

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