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|Title: ||Analysis of aptamer sequence activity relationships|
|Authors: ||Platt, Mark|
Day, Philip J.R.
Kell, Douglas B.
|Keywords: ||Cell Biology|
|Issue Date: ||2009|
|Publisher: ||© Royal Society of Chemistry|
|Citation: ||PLATT, M., ROWE, W., KNOWLES, J. ... et al, 2009. Analysis of aptamer sequence activity relationships. Integrative Biology, 1 (1), pp.116-122.|
|Abstract: ||DNA sequences that can bind selectively and specifically to target molecules are known as
aptamers. Normally such binding analyses are performed using soluble aptamers. However, there
is much to be gained by using an on-chip or microarray format, where a large number of
aptameric DNA sequences can be interrogated simultaneously. To calibrate the system, known
thrombin binding aptamers (TBAs) have been mutated systematically, producing large
populations that allow exploration of key structural aspects of the overall binding motif. The
ability to discriminate between background noise and low affinity binding aptamers can be
problematic on arrays, and we use the mutated sequences to establish appropriate experimental
conditions and their limitations for two commonly used fluorescence-based detection methods.
Having optimized experimental conditions, high-density oligonucleotide microarrays were used to
explore the entire loop–sequence–functionality relationship creating a detailed model based on
over 40 000 analyses, describing key features for quadruplex-forming sequences.|
|Description: ||This article is closed access.|
|Version: ||Closed access|
|Publisher Link: ||http://dx.doi.org/10.1039/b814892a|
|Appears in Collections:||Closed Access (Chemistry)|
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