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Convergent evolution to an aptamer observed in small populations on DNA microarrays

journal contribution
posted on 2013-01-02, 12:37 authored by William Rowe, Mark PlattMark Platt, David C. Wedge, Philip J.R. Day, Douglas B. Kell, Joshua Knowles
The development of aptamers on custom synthesized DNA microarrays, which has been demonstrated in recent publications, can facilitate detailed analyses of sequence and fitness relationships. Here we use the technique to observe the paths taken through sequence-fitness space by three different evolutionary regimes: asexual reproduction, recombination and model-based evolution. The different evolutionary runs are made on the same array chip in triplicate, each one starting from a small population initialized independently at random. When evolving to a common target protein, glucose-6-phosphate dehydrogenase (G6PD), these nine distinct evolutionary runs are observed to develop aptamers with high affinity and to converge on the same motif not present in any of the starting populations. Regime specific differences in the evolutions, such as speed of convergence, could also be observed.

History

School

  • Science

Department

  • Chemistry

Citation

ROWE, W., PLATT, M., WEDGE, D.C. ... et al, 2012. Convergent evolution to an aptamer observed in small populations on DNA microarrays. Physical Biology, 7(3), 036007.

Publisher

© IOP Publishing

Version

  • VoR (Version of Record)

Publication date

2010

Notes

This article is closed access.

ISSN

1478-3967

Language

  • en

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