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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/11330

Title: M13 bacteriophage-activated superparamagnetic beads for affinity separation
Authors: Muzard, Julien
Platt, Mark
Lee, Gil U.
Keywords: Bionanomaterial
M13 filamentous phage
Affinity separation
Downstream processing
Superparamagnetic bead
High gradient magnetic separation
Issue Date: 2012
Publisher: © WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Citation: MUZARD, J., PLATT, M. and LEE, G.U., 2012. M13 bacteriophage-activated superparamagnetic beads for affinity separation. Small, 8 (15), pp.2403-2411.
Abstract: The growth of the biopharmaceutical industry has created a demand for new technologies for the purification of genetically engineered proteins. The efficiency of large-scale, highgradient magnetic fishing could be improved if magnetic particles offering higher binding capacity and magnetization were available. This article describes several strategies for synthesizing SPMs that are composed of a M13 bacteriophage layer assembled on a superparamagnetic core. Chemically cross-linking the pVIII proteins to a carboxyl functionalized SPM produced highly responsive superparamagnetic particles with a side-on oriented, adherent virus monolayer. Also, the genetic manipulation of the pIII proteins with a His6 peptide sequence allowed reversible assembly of the bacteriophage on a nitrilotriacetic acid functionalized core in an end-on configuration. These phage-magnetic particles were successfully used to separate antibodies from high-protein concentration solutions in a single step with a > 90 % purity. The dense magnetic core of these particles makes them five times more responsive to magnetic fields than commercial materials composed of polymer-iron oxide composites and a monolayer of phage could produced a 1000 fold higher antibody binding capacity. These new bionanomaterials appear to be well-suited to large-scale highgradient magnetic fishing separation and promise to be cost effective as a result of the selfassembling and self-replicating properties of genetically engineered M13 bacteriophage.
Description: This article was submitted for publication in the journal, Small. It is the pre-peer reviewed version of the article.
Version: Submitted for publication
DOI: 10.1002/smll.201290083
URI: https://dspace.lboro.ac.uk/2134/11330
Publisher Link: http://dx.doi.org/10.1002/smll.201290083
ISSN: 1613-6810
Appears in Collections:Published Articles (Chemistry)

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