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|Title: ||Eicosapentaenoic acid is more effective than docosahexaenoic acid in inhibiting proinflammatory mediator production and transcription from LPS-induced human asthmatic alveolar macrophage cells|
|Authors: ||Mickleborough, Timothy D.|
Tecklenburg, Sandra L.
Montgomery, Gregory S.
Lindley, Martin R.
|Keywords: ||Eicosapentaenoic acid|
|Issue Date: ||2009|
|Publisher: ||© Elsevier Ltd and European Society for Clinical Nutrition and Metabolism|
|Citation: ||MICKLEBOROUGH, T.D. ... et al., 2009. Eicosapentaenoic acid is more effective than docosahexaenoic acid in inhibiting proinflammatory mediator production and transcription from LPS-induced human asthmatic alveolar macrophage cells. Clinical Nutrition, 28 (1), pp. 71 - 77.|
|Abstract: ||Background & aims: The purpose of the study was to determine which of the active constituents of fish
oil, eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA), is most effective in suppressing proinflammatory
mediator generation and cytokine expression from LPS-stimulated human asthmatic
alveolar macrophages (AMΦ).
Methods: The AMΦ were obtained from twenty-one asthmatic adults using fiberoptic bronchoscopy.
Cells were pretreated with DMEM, pure EPA, an EPA-rich media (45% EPA/10% DHA), pure DHA, a DHArich
media (10% EPA/50% DHA) or Lipovenos® (n-6 PUFA), and then exposed to Dulbecco’s Modified
Eagle’s Medium (DMEM) (-) or LPS (+). Supernatants were analyzed for leukotriene (LT)B4, prostaglandin
(PG)D2, tumor necrosis factor (TNF)-α and interleukin (IL)-1β production. Detection of TNF-α and
IL-1β mRNA expression levels was quantified by reverse transcriptase polymerase chain reaction.
Results: 120 μM pure EPA and EPA-rich media significantly (p < 0.05) suppressed TNF-a and IL-1b mRNA
expression and the production of LTB4, PGD2 and TNF-a and IL-1b in LPS-stimulated primary AMφ cells
obtained from asthmatic patients to a much greater extent than 120 mM pure DHA and DHA-rich media
Conclusions: This study has shown for the first time that EPA is a more potent inhibitor than DHA of
inflammatory responses in human asthmatic AMΦ cells.|
|Description: ||This paper was published in the journal, Clinical Nutrition [© Elsevier Ltd and European Society for Clinical Nutrition and Metabolism] and the definitive version is available at: http://dx.doi.org/10.1016/j.clnu.2008.10.012|
|Version: ||Accepted for publication|
|Publisher Link: ||http://dx.doi.org/10.1016/j.clnu.2008.10.012|
|Appears in Collections:||Published Articles (Sport, Exercise and Health Sciences)|
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