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Title: Loss of solubility of alpha-lactalbumin and beta-lactoglobulin during the spray drying of whey proteins
Authors: Anandharamakrishnan, C.
Rielly, Chris D.
Stapley, A.G.F.
Keywords: Reversed phase HPLC
Feed concentration
Outlet temperature
Denaturation
Aggregation
Milk
Issue Date: 2008
Publisher: © Elsevier
Citation: ANANDHARAMAKRISHNAN, C., RIELLY, C.D. and STAPLEY, A.G.F., 2008. Loss of solubility of alpha-lactalbumin and beta-lactoglobulin during the spray drying of whey proteins. LWT - Food Science and Technology, 41 (2), pp. 270-277.
Abstract: A reversed phase HPLC technique (at pH 4.6) has been developed to measure 10 the loss of solubility of α-lactalbumin and β-lactoglobulin resulting from the 11 spray drying of whey protein isolate solution. Spray drying was performed in a 12 pilot-scale co-current spray dryer with different feed concentrations (20 - 40% 13 w/v) and outlet temperatures (60°C to 120°C). The study reveals that the 14 solubility of both α-lactalbumin and β-lactoglobulin was not significantly affected 15 at low outlet gas temperatures (60-80°C), but was strongly affected (up to 40%) 16 at high temperatures (100-120°C). Significantly higher losses in solubility were 17 observed for β-lactoglobulin compared to α-lactalbumin. Increasing the feed 18 concentration at higher outlet temperatures also caused noticeable increases in 19 insolubility. The reversed phase HPLC results were consistent with those from 20 total protein nitrogen content (Kjeldhal) analysis.
Description: This is the author’s version of a work that was accepted for publication in the LWT - Food Science and Technology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published at: http://dx.doi.org/10.1016/j.lwt.2007.03.004
Version: Accepted for publication
DOI: 10.1016/j.lwt.2007.03.004
URI: https://dspace.lboro.ac.uk/2134/11457
Publisher Link: http://dx.doi.org/10.1016/j.lwt.2007.03.004
ISSN: 0023-6438
Appears in Collections:Published Articles (Chemical Engineering)

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