The chromatographic behaviour of a new column packing for high
performance size exclusion chromatography based on crosslinked
polyacrylamide particles was studied. Experimental retention data for
poly(ethylene glycol)and poly(ethylene oxide) standards in water and
water-methanol mixtures showed that the separation mechanism is size
exclusion, since a universal calibration plot based on hydrodynamic volume
was obtained. For polysaccharide standards in water and water-methanol
(80/20), the universal calibration was valid for molecular weights above
4 * 10~. Below this value, secondary mechanisms appear to be taking part
in the separation since water-methanol (80/20) is a poor solvent for
polysaccharides. Crosslinked polyacrylamide packings showed an interactive
behaviour with tetrahydrofuran and dimethylformamide as eluents with
polystyrene and poly( ethylene glycol )/poly(ethylene oxide) standards
and the universal calibration was not valid....
A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy of Loughborough University.