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|Title: ||The effect of a paced auditory serial addition test (PASAT) intervention on the profile of volatile organic compounds in human breath: a pilot study|
|Authors: ||Turner, Matthew A.|
Martin, Helen J.
Wilson, Ian D.
Thomas, C.L. Paul
|Keywords: ||Breath analysis|
|Issue Date: ||2013|
|Publisher: ||© IOP Publishing Ltd|
|Citation: ||TURNER, M.A. ... et al., 2013. The effect of a paced auditory serial addition test (PASAT) intervention on the profile of volatile organic compounds in human breath: a pilot study. Journal of Breath Research, 7 (1), 11pp.|
|Abstract: ||This study sought to identify if detectable changes in human breath profiles may be observed following a psychological intervention designed to induce stress, a paced auditory serial addition test (PASAT). Breath samples were collected from 22 participants (10 male and 12 female) following a double cross-over randomized design with two experimental interventions. One intervention required participants to listen to classical music chosen to be neutral. The other intervention required participants to undertake a PASAT that induced cardiovascular responses consistent with acute stress. Both interventions also involved two sequences of cognitive function tests. Blood-pressure and heart-rate were recorded throughout each intervention and distal breath samples were collected onto Tenax® TA/Carbograph 1 thermal desorption tubes, using an adaptive breath sampler. Samples were collected before and after the PASAT. Breath samples were analysed by thermal desorption gas chromatography-mass spectrometry. Data registration using retention indexing and peak deconvolution followed by partial least-squares discriminant analysis identified six stress sensitive compounds. A principal components analysis model based on these components generated a model that predicted post-PASAT versus post-neutral intervention samples with a sensitivity of 83.3% and a selectivity of 91.6% for females, compared to 100% sensitivity and 90% selectivity for males. Of the six compounds indole, 2-hydroxy-1-phenylethanone, benzaldehyde, and 2-ethylhexan-1-ol were identified on the basis of mass spectral, retention indexing and confirmation against pure standards. 2-methylpentadecane was tentatively identified from mass spectral and retention indexing, whilst one component has yet to be assigned, although the mass spectrum is indicative of a terpene. Indole and 2-methylpentadecane concentrations increased in response to the PASAT intervention, while the other compounds reduced in their abundance in human breath, possibly as a result of ventilation effects.|
|Description: ||This article was published in the Journal of Breath Research [© IOP Publishing] and the definitive version is available at: http://dx.doi.org/10.1088/1752-7155/7/1/017102|
|Version: ||Accepted for publication|
|Publisher Link: ||http://dx.doi.org/10.1088/1752-7155/7/1/017102|
|Appears in Collections:||Published Articles (Chemistry)|
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