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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/13177

Title: A novel automated bioreactor for scalable process optimisation of haematopoietic stem cell culture
Authors: Ratcliffe, Elizabeth
Glen, Katie E.
Workman, Victoria L.
Stacey, Adrian J.
Thomas, Robert James
Keywords: Haematopoietic stem cells
Suspension culture
Cell culture automation
Process development
Issue Date: 2012
Publisher: © Elsevier B.V.
Citation: RATCLIFFE, E. ... et al, 2012. A novel automated bioreactor for scalable process optimisation of haematopoietic stem cell culture. Journal of Biotechnology, 161 (3), pp. 387 - 390.
Abstract: Proliferation and differentiation of haematopoietic stem cells (HSCs) from umbilical cord blood at large scale will potentially underpin production of a number of therapeutic cellular products in development, including erythrocytes and platelets. However, to achieve production processes that are scalable and optimised for cost and quality, scaled down development platforms that can define process parameter tolerances and consequent manufacturing controls are essential. We have demonstrated the potential of a new, automated, 24 × 15 mL replicate suspension bioreactor system, with online monitoring and control, to develop an HSC proliferation and differentiation process for erythroid committed cells (CD71+, CD235a+). Cell proliferation was relatively robust to cell density and oxygen levels and reached up to 6 population doublings over 10 days. The maximum suspension culture density for a 48 h total media exchange protocol was established to be in the order of 107 cells/mL. This system will be valuable for the further HSC suspension culture cost reduction and optimisation necessary before the application of conventional stirred tank technology to scaled manufacture of HSC derived products.
Description: This article was publised in the serial, Journal of Biotechnology [© Elsevier B.V.]. The definitive version is available at: http://dx.doi.org/10.1016/j.jbiotec.2012.06.025
Version: Accepted for publication
DOI: 10.1016/j.jbiotec.2012.06.025
URI: https://dspace.lboro.ac.uk/2134/13177
Publisher Link: http://dx.doi.org/10.1016/j.jbiotec.2012.06.025
ISSN: 0168-1656
Appears in Collections:Published Articles (Mechanical, Electrical and Manufacturing Engineering)

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