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Incorporation of hydroxyapatite sol into collagen gel to regulate the contraction mediated by human bone marrow-derived stromal cells
In this paper, hydroxyapatite (HA) sol composed of
nanosized HA particles was incorporated into collagen gel to regulate
cell-mediated collagen contraction. Human bone marrow derived
stromal cells (hMSCs) were cultured for 37 days in gels with
HA to collagen ratios of 0:1, 0.5:1, 1:1, 1.5:1, and 2:1, respectively.
The contraction of gels was evaluated by measuring diameter
change with incubation time, and the microstructure of gels
at the end of culture was visualized by SEM. The cytotoxicity of
HA sol was evaluated according to the percentage of maximum
lactate dehydrogenase (LDH) release from hMSCs. A combination
of Hoechst 33342, propidium iodide, and calcein esters was used
for imaging the live/dead cells and the distribution of loaded cells
within the constructs. By incorporating HA sol into the collagen
gel, the hMSCs medicated contraction was delayed and the extent
of contraction was reduced as the proportion of HA relative to the
collagen increased. Depending on the ratio of HA sol incorporated
within the collagen gel, hMSC cells revealed different morphology
and a change in the distribution of loaded cells within the gels. No
significant cytotoxicity was noticed as a consequence of incorporating
HA sol into the collagen lattice.
Funding
This work was supported by the U.K. Engineering and Physical Sciences Research Council funded Innovative Manufacturing Grand Challenge in Regenerative Medicine, remedi, a partnership of Loughborough, Nottingham, Cambridge, Birmingham, Ulster and Liverpool universities, and industry and agency stakeholders. The work of Y. Liu was supported by a Research Councils United Kingdom fellowship.
History
School
- Mechanical, Electrical and Manufacturing Engineering
Citation
LIU, Y. and WILLIAMS, D.J., 2010. Incorporation of hydroxyapatite sol into collagen gel to regulate the contraction mediated by human bone marrow-derived stromal cells. IEEE Transactions on Nanobioscience, 9 (1), pp.1-11.Publisher
© IEEEVersion
- VoR (Version of Record)
Publication date
2010Notes
This article is closed access.ISSN
1536-1241Publisher version
Language
- en