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Title: Changes in the stiffness of human mesenchymal stem cells with the progress of cell death as measured by atomic force microscopy
Authors: Nikolaev, N.I.
Muller, Torsten
Williams, David J.
Liu, Yang
Keywords: Atomic Force Microscopy (AFM)
Stem cells
Elastic modulus
Cell death
Issue Date: 2013
Publisher: © The Authors. Published by Elsevier Ltd.
Citation: NIKOLAEV, N.I. ... et al., 2013. Changes in the stiffness of human mesenchymal stem cells with the progress of cell death as measured by atomic force microscopy. Journal of Biomechanics, 47 (3), pp. 625–630.
Abstract: This note reports observations of the change of stiffness of human mesenchymal stem cells (hMSCs) with the progress of cell death as measured by AFM. hMSC with impaired membrane, dead and viable cells were labelled with Annexin V and Propidium Iodide after 24 h cold storage, followed by AFM measurement and Young's modulus of cells was derived. Viable hMSCs have a Young's modulus (E) in the range of 0.81-1.13 kPa and consistent measurement was observed when different measurement locations were chosen. E of cells with partially impaired membrane was 0.69±0.17 kPa or in the range of 2.04-4.74 kPa, depending upon the measurement locations. With the loss of membrane integrity, though there was no variation on measured E between different locations, a mixed picture of cell stiffness was observed as indicated by cells with E as low as 0.09±0.03 kPa, in a mid-range of 4.62±0.67 kPa, and the highest of up to 48.98±19.80 kPa. With the progress of cell death, the highest stiffness was noticed for cells showing a more granular appearance; also the lowest stiffness for cells with vacuole appearance. Findings from this study indicate that cell stiffness is significantly altered with the progress of cell death. © 2013 The Authors.
Description: This is an Open Access Article. It is published by Elsevier under the Creative Commons Attribution 3.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/
Sponsor: Dr. N. Nikolaev was supported by the TSB, EPSRC and Loughborough University. Dr. Y. Liu was additionally supported by a UK Research Council Fellowship, Royal Society, FP7-PEOPLE-2012- IRSES (SkelGen) and EPSRC Centre for Innovative Manufacturing in Regenerative Medicine. EPSRC [grant numbers ECP020/0811 and EP/H028277/1].
Version: Published
DOI: 10.1016/j.jbiomech.2013.12.004
URI: https://dspace.lboro.ac.uk/2134/14024
Publisher Link: http://dx.doi.org/10.1016/j.jbiomech.2013.12.004
ISSN: 0021-9290
Appears in Collections:Published Articles (Mechanical, Electrical and Manufacturing Engineering)

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