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Title: Production of liposomes using microengineered membrane and co-flow microfluidic device
Authors: Vladisavljevic, Goran T.
Laouini, Abdallah
Charcosset, Catherine
Fessi, Hatem
Bandulasena, Hemaka C.H.
Holdich, R.G.
Keywords: Liposome
Laminar co-flow
CFD simulation
Microfluidic mixer
Membrane dispersion
Issue Date: 2014
Publisher: © Elsevier
Citation: VLADISAVLJEVIC, G.T. ... et al, 2014. Production of liposomes using microengineered membrane and co-flow microfluidic device. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 458, pp. 168-177.
Abstract: Two modified ethanol injection methods have been used to produce Lipoid® E80 and POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) liposomes: (i) injection of the organic phase through a microengineered nickel membrane kept under controlled shear conditions and (ii) injection of the organic phase through a tapered-end glass capillary into co-flowing aqueous stream using coaxial assemblies of glass capillaries. The organic phase was composed of 20 mg ml−1 of phospholipids and 5 mg ml−1 of cholesterol dissolved in ethanol and the aqueous phase was ultra-pure water. Self-assembly of phospholipid molecules into multiple concentric bilayers via phospolipid bilayered fragments was initiated by interpenetration of the two miscible solvents. The mean vesicle size in the membrane method was 80 ± 3 nm and consistent across all of the devices (stirred cell, cross-flow module and oscillating membrane system), indicating that local or temporal variations of the shear stress on the membrane surface had no effect on the vesicle size, on the condition that a maximum shear stress was kept constant. The mean vesicle size in co-flow microfludic device decreased from 131 to 73 nm when the orifice diameter in the injection capillary was reduced from 209 to 42 μm at the aqueous and organic phase flow rate of 25 and 5.55 ml h−1, respectively. The vesicle size was significantly affected by the mixing efficiency, which was controlled by the orifice size and liquid flow rates. The smallest vesicle size was obtained under conditions that promote the highest mixing rate. Computational Fluid Dynamics (CFD) simulations were performed to study the mixing process in the vicinity of the orifice.
Description: NOTICE: this is the author’s version of a work that was accepted for publication in Colloids and Surfaces A: Physicochemical and Engineering Aspects. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol 458, 2014, DOI:10.1016/j.colsurfa.2014.03.016
Version: Accepted for publication
DOI: 10.1016/j.colsurfa.2014.03.016
URI: https://dspace.lboro.ac.uk/2134/14323
Publisher Link: http://dx.doi.org/10.1016/j.colsurfa.2014.03.016
ISSN: 0927-7757
Appears in Collections:Published Articles (Chemical Engineering)

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