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Title: Kinetics of the pre-treatment of used cooking oil using Novozyme 435 for biodiesel production
Authors: Haigh, Kathleen F.
Vladisavljevic, Goran T.
Reynolds, James C.
Nagy, Zoltan K.
Saha, Basu
Keywords: Biodiesel
Liquid chromatography
Used cooking oil
Novozyme 435
Kinetic model
Fatty acid methyl esters (FAMEs)
Ping Pong Bi Bi mechanism
Issue Date: 2014
Publisher: Elsevier / © The Institution of Chemical Engineers
Citation: HAIGH, K.F. ... et al, 2014. Kinetics of the pre-treatment of used cooking oil using Novozyme 435 for biodiesel production. Chemical Engineering Research and Design, 92 (4), pp. 713–719.
Abstract: The pre-treatment of used cooking oil (UCO) for the preparation of biodiesel has been investigated using Novozyme 435, Candida antarctica Lipase B immobilised on acrylic resin, as the catalyst. The reactions in UCO were carried out using a jacketed batch reactor with a reflux condenser. The liquid chromatography–mass spectrometry (LC–MS) method was developed to monitor the mono-, di- and triglyceride concentrations and it was found that the method was sensitive enough to separate isomers, including diglyceride isomers. It was found that the 1,3 diglyceride isomer reacted more readily than the 1,2 isomer indicating stereoselectivity of the catalyst. This work showed that Novozyme 435 will catalyse the esterification of free fatty acids (FFAs) and the transesterification of mono- and diglycerides typically found in UCO when Novozyme 435 is used to catalyse the pre-treatment of UCO for the formation of biodiesel. A kinetic model was used to investigate the mechanism and indicated that the reaction progressed with the sequential hydrolysis esterification reactions in parallel with transesterification.
Description: This is an Open Access Article. It is published by Elsevier under the Creative Commons Attribution 3.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/
Sponsor: We would like to thank EPSRC for the PhD scholarship to KH. We would also like to thank GreenFuel Oil Co., Ltd., UK for supplying the UCO and Novozymes UK Ltd. (Dr. David Cowan) for supplying the enzyme catalyst and his help and advice with using Novozyme 435 for this project.
Version: Published
DOI: 10.1016/j.cherd.2014.01.006
URI: https://dspace.lboro.ac.uk/2134/14433
Publisher Link: http://dx.doi.org/10.1016/j.cherd.2014.01.006
ISSN: 0263-8762
Appears in Collections:Published Articles (Chemical Engineering)

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