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Synthesis and characterization of size-selective nanoporous polymeric adsorbents for blood purification
journal contribution
posted on 2014-06-05, 12:46 authored by Danish MalikDanish Malik, Chris Webb, Richard Holdich, J.J. Ramsden, G.L. Warwick, Iain Roche, David Williams, Andrzej W. Trochimczuk, J.A. Dale, N.A. HoenichCrosslinked polystyrene co-divinylbenzene adsorbent microspheres with median diameters between 40 and 300 μm, with an enhanced proportion of mesopores in the range 4–10 nm, have been synthesized using a membrane emulsification technique. The aim was to develop an adsorbent for the selective removal of middle molecular weight uraemic toxins (size range 0.5–20 kDa), e.g. β2-microglobulin (11.8 kDa), whilst size-excluding larger blood proteins, e.g. serum albumin (MW 69 kDa). Using inverse size exclusion chromatography, the role of size exclusion has been demonstrated using hen egg lysozyme (a surrogate for β2-microglobulin) and human serum albumin as model proteins for batch adsorption studies. The adsorbent sample possessing a nanoporous structure with pores predominantly smaller than 10 nm successfully size-excludes serum albumin whilst displaying significant adsorption capacity for the middle molecular weight lysozyme (14.3 kDa).
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
SEPARATION AND PURIFICATION TECHNOLOGYVolume
66Issue
3Pages
578 - 585 (8)Citation
MALIK, D.J. ... et al., 2009. Synthesis and characterization of size-selective nanoporous polymeric adsorbents for blood purification. Separation and Purification Technology, 66 (3), pp. 578 - 585.Publisher
© Elsevier B.V.Version
- VoR (Version of Record)
Publication date
2009Notes
Closed access. This article was published in the journal, Separation and Purification Technology [© Elsevier B.V.]ISSN
1383-5866Publisher version
Language
- en