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|Title: ||The physical characterisation of a microscale parallel bioreactor platform with an industrial CHO cell line expressing an IgG4|
|Authors: ||Nienow, Alvin W.|
Rielly, Chris D.
Hewitt, Christopher J.
|Keywords: ||Ambr (TM) microscale bioreactor|
CHO cell culture
Specific power input
|Issue Date: ||2013|
|Publisher: ||© Elsevier B.V|
|Citation: ||NIENOW, A.W. ... et al., 2013. The physical characterisation of a microscale parallel bioreactor platform with an industrial CHO cell line expressing an IgG4. Biochemical Engineering Journal, 76, pp. 25 - 36.|
|Abstract: ||There is a growing body of evidence that the ambrTM workstation from TAP Biosystems performs well
in terms of helping to select appropriate clones for scale-up studies. Here we have investigated the
physical characteristics of this microscale bioreactor system and found that these are quite different from
those that exist in larger scale stirred bioreactors. For example, the ﬂow regime in the ambrTM vessel is
transitional rather than turbulent and the sparged air/oxygen superﬁcial gas velocity is relatively very low
whilst the speciﬁc power input is much higher (∼400 W/m3) when compared to that used at larger scales
(typically ∼20 W/m3). This speciﬁc power input is necessary in order to achieve kLa values sufﬁciently
high to satisfy the oxygen demand of the cells and control of dO2. In line with other studies, we ﬁnd
that the culture of CHO cells in a 15 mL ambrTM bioreactor gave similar cell growth and productivity to
that achieved in a 5 L stirred bioreactor whilst the results from shake ﬂasks were signiﬁcantly different.
Given the differences in physical characteristics between the ambrTM and larger stirred bioreactors, we
suggest that this similarity in biological performance is due to their similar control capabilities and the
‘equivalence of the stress parameters’ across the scales when compared with shake ﬂasks.|
|Description: ||This article was published in the Biochemical Engineering Journal [© Elsevier B.V.] and the definitive version is available at: http://dx.doi.org/10.1016/j.bej.2013.04.011|
|Version: ||Submitted for publication|
|Publisher Link: ||http://dx.doi.org/10.1016/j.bej.2013.04.011|
|Appears in Collections:||Published Articles (Chemical Engineering)|
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