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Title: The physical characterisation of a microscale parallel bioreactor platform with an industrial CHO cell line expressing an IgG4
Authors: Nienow, Alvin W.
Rielly, Chris D.
Brosnan, Kathryn
Bargh, Neil
Lee, Kenneth
Coopman, Karen
Hewitt, Christopher J.
Keywords: Ambr (TM) microscale bioreactor
CHO cell culture
Transitional flow
Specific power input
Mass transfer
Issue Date: 2013
Publisher: © Elsevier B.V
Citation: NIENOW, A.W. ... et al., 2013. The physical characterisation of a microscale parallel bioreactor platform with an industrial CHO cell line expressing an IgG4. Biochemical Engineering Journal, 76, pp. 25 - 36.
Abstract: There is a growing body of evidence that the ambrTM workstation from TAP Biosystems performs well in terms of helping to select appropriate clones for scale-up studies. Here we have investigated the physical characteristics of this microscale bioreactor system and found that these are quite different from those that exist in larger scale stirred bioreactors. For example, the flow regime in the ambrTM vessel is transitional rather than turbulent and the sparged air/oxygen superficial gas velocity is relatively very low whilst the specific power input is much higher (∼400 W/m3) when compared to that used at larger scales (typically ∼20 W/m3). This specific power input is necessary in order to achieve kLa values sufficiently high to satisfy the oxygen demand of the cells and control of dO2. In line with other studies, we find that the culture of CHO cells in a 15 mL ambrTM bioreactor gave similar cell growth and productivity to that achieved in a 5 L stirred bioreactor whilst the results from shake flasks were significantly different. Given the differences in physical characteristics between the ambrTM and larger stirred bioreactors, we suggest that this similarity in biological performance is due to their similar control capabilities and the ‘equivalence of the stress parameters’ across the scales when compared with shake flasks.
Description: This article was published in the Biochemical Engineering Journal [© Elsevier B.V.] and the definitive version is available at: http://dx.doi.org/10.1016/j.bej.2013.04.011
Version: Submitted for publication
DOI: 10.1016/j.bej.2013.04.011
URI: https://dspace.lboro.ac.uk/2134/14777
Publisher Link: http://dx.doi.org/10.1016/j.bej.2013.04.011
ISSN: 1369-703X
Appears in Collections:Published Articles (Chemical Engineering)

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