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Title: Scale-down studies for assessing the impact of different stress parameters on growth and product quality during animal cell culture
Authors: Nienow, Alvin W.
Scott, William H.
Hewitt, Christopher J.
Thomas, Colin R.
Lewis, Gareth
Amanullah, Ashraf
Kiss, Robert
Meier, Steven J.
Keywords: CHO cells
Mechanical stress
Kolmogorov scale of turbulence
Environmental stress
Product quality
Glycosolation
Process performance
Issue Date: 2013
Publisher: Elsevier / © The Institution of Chemical Engineers
Citation: NIENOW, A.W. ... et al, 2013. Scale-down studies for assessing the impact of different stress parameters on growth and product quality during animal cell culture. Chemical Engineering Research and Design, 91 (11), pp. 2265 - 2274
Abstract: Two series of reproducible fed-batch bench scale cultures have been undertaken, one series simulating the impact of spatial variations in pH and nutrients as found at commercial scale on performance, the other, the impact of fluid dynamic stresses associated with agitation. The first was unsuccessful because, somewhat surprisingly, the use of a peristaltic pump to circulate cells and medium through different spatial environments always led to a similar reduction in culture time and resulting product titre compared to uncirculated controls. This fall was sufficient to essentially mask other effects. In the second, even at maximum specific energy dissipation rates up to ~160 times > with laminar extensional flow and ~25 times > with turbulent flow compared to typical commercial conditions, no significant effects were observed on cell growth and viability. Most importantly, in all of the cases studied, product quality was unaffected compared to controls. In addition, it is suggested that because of the possibility of cell line specific behaviour and the relationship between damage to entities and the Kolmogorov scale of turbulence, sensitivity to fluid dynamic stresses is best studied in turbulent bench scale bioreactors. © 2013 The Institution of Chemical Engineers.
Description: This is the author’s version of a work that was accepted for publication in Chemical Engineering Research and Design. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Chemical Engineering Research and Design, 91 (11), pp. 2265 - 2274, DOI: 10.1016/j.cherd.2013.04.002
Version: Accepted for publication
DOI: 10.1016/j.cherd.2013.04.002
URI: https://dspace.lboro.ac.uk/2134/14846
Publisher Link: http://dx.doi.org/10.1016/j.cherd.2013.04.002
ISSN: 0263-8762
Appears in Collections:Published Articles (Chemical Engineering)

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