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Title: The effect of interleukin-6 and the interleukin-6 receptor on glucose transport in mouse skeletal muscle
Authors: Gray, Stuart R.
Ratkevicius, Aivaras
Wackerhage, Henning
Coats, Paul
Nimmo, Myra A.
Issue Date: 2009
Publisher: © The Authors. Journal compilation © The Physiological Society
Citation: GRAY, S.R. ... et al., 2009. The effect of interleukin-6 and the interleukin-6 receptor on glucose transport in mouse skeletal muscle. Experimental Physiology, 94 (8), pp. 899 - 905.
Abstract: Exercise results in an increase in interleukin-6 (IL-6), its receptor (IL-6R) and skeletal muscle glucose transport. Interleukin-6 has been found to have equivocal effects on glucose transport, with no studies, to our knowledge, investigating any potential role of IL-6R. In the present study, we hypothesized that a combined preparation of IL-6 and soluble IL-6R (sIL-6R) would stimulate glucose transport. Mouse soleus muscles were incubated with physiological and supraphysiological concentrations of IL-6 and a combination of IL-6 and sIL-6R. Total and phosphorylated AMP-activated protein kinase (AMPK) and Protein Kinase B (PKB/Akt) were also measured by Western blotting. Exposure to both physiological (80 pg ml−1) and supraphysiological IL-6 (120 ng ml−1) had no effect on glucose transport. At physiological levels, exposure to a combination of IL-6 and sIL-6R (32 ng ml−1) resulted in a 1.4-fold increase (P < 0.05) in basal glucose transport with no change to the phosphorylation of AMPK. Exposure to supraphysiological levels of IL-6 and sIL-6R (120 ng ml−1) resulted in an approximately twofold increase (P < 0.05) in basal glucose transport and an increase (P < 0.05) in AMPK phosphorylation. No effect of IL-6 or sIL-6R was observed on insulin-stimulated glucose transport. These findings demonstrate that, while IL-6 alone does not stimulate glucose transport in mouse soleus muscle, when sIL-6R is introduced glucose transport is directly stimulated, partly through AMPK-dependent signalling.
Description: Closed access.
Version: Published
DOI: 10.1113/expphysiol.2009.048173
URI: https://dspace.lboro.ac.uk/2134/15162
Publisher Link: http://dx.doi.org/10.1113/expphysiol.2009.048173
ISSN: 0958-0670
Appears in Collections:Closed Access (Sport, Exercise and Health Sciences)

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