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Title: Probing biomolecular interaction forces using an anharmonic acoustic technique for selective detection of bacterial spores
Authors: Ghosh, Sourav K.
Ostanin, Victor P.
Johnson, Christian L.
Lowe, Christopher R.
Seshia, Ashwin A.
Keywords: Nonlinear acoustics
Anharmonic interaction
Label-free detection
Force spectroscopy
Bacillus subtilis
Issue Date: 2011
Publisher: © Elsevier
Citation: GHOSH, S.K. ... et al, 2011. Probing biomolecular interaction forces using an anharmonic acoustic technique for selective detection of bacterial spores. Biosensors and Bioelectronics, 29 (1), pp. 145 - 150
Abstract: Receptor-based detection of pathogens often suffers from non-specific interactions, and as most detection techniques cannot distinguish between affinities of interactions, false positive responses remain a plaguing reality. Here, we report an anharmonic acoustic based method of detection that addresses the inherent weakness of current ligand dependant assays. Spores of Bacillus subtilis (Bacillus anthracis simulant) were immobilized on a thickness-shear mode AT-cut quartz crystal functionalized with anti-spore antibody and the sensor was driven by a pure sinusoidal oscillation at increasing amplitude. Biomolecular interaction forces between the coupled spores and the accelerating surface caused a nonlinear modulation of the acoustic response of the crystal. In particular, the deviation in the third harmonic of the transduced electrical response versus oscillation amplitude of the sensor (signal) was found to be significant. Signals from the specifically-bound spores were clearly distinguishable in shape from those of the physisorbed streptavidin-coated polystyrene microbeads. The analytical model presented here enables estimation of the biomolecular interaction forces from the measured response. Thus, probing biomolecular interaction forces using the described technique can quantitatively detect pathogens and distinguish specific from non-specific interactions, with potential applicability to rapid point-of-care detection. This also serves as a potential tool for rapid force-spectroscopy, affinity-based biomolecular screening and mapping of molecular interaction networks. © 2011 Elsevier B.V.
Description: This is the author’s version of a work that was accepted for publication in Biosensors and Bioelectronics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biosensors and Bioelectronics, 29 (1), 2011, DOI: 10.1016/j.bios.2011.08.008
Sponsor: This project was funded in part by the UK Engineering and Physical Sciences Research Council and the Cambridge Trusts.
Version: Accepted for publication
DOI: 10.1016/j.bios.2011.08.008
URI: https://dspace.lboro.ac.uk/2134/15789
Publisher Link: http://dx.doi.org/10.1016/j.bios.2011.08.008
ISSN: 0956-5663
Appears in Collections:Published Articles (Mechanical, Electrical and Manufacturing Engineering)

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