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Title: Anharmonic surface interactions for biomolecular screening and characterization
Authors: Ghosh, Sourav K.
Ostanin, Victor P.
Seshia, Ashwin A.
Issue Date: 2011
Publisher: © American Chemical Society
Citation: GHOSH, S.K., OSTANIN, V.P. and SESHIA, A.A., 2011. Anharmonic surface interactions for biomolecular screening and characterization. Analytical Chemistry, 83 (2), pp. 549 - 554
Abstract: The acoustic response of conventional mechanical oscillators, such as a piezoelectric crystal, is predominantly harmonic at modest amplitudes. However, here, we observe from the electrical response that significant motional anharmonicity is introduced in the presence of attached analyte. Experiments were conducted with streptavidin-coated polystyrene microbeads of various sizes attached to a quartz crystal resonator via specific and nonspecific molecular tethers in liquid. Quantitative analysis reveals that the deviation of odd Fourier harmonics of the response caused by introduction of microbeads as a function of oscillation amplitude presents a unique signature of the molecular tether. Hence, the described anharmonic detection technique (ADT) based on this function allows screening of biomolecules and provides an additional level of selectivity in receptor-based detection that is often associated with nonspecific interactions. We also propose methods to extract mechanical force-extension characteristics of the molecular tether and activation energy using this technique. © 2010 American Chemical Society.
Description: This article is closed access.
Sponsor: This project was funded in part by the Engineering and Physical Sciences Research Council and the Cambridge Commonwealth Trust.
Version: Accepted for publication
DOI: 10.1021/ac102261q
URI: https://dspace.lboro.ac.uk/2134/15792
Publisher Link: http://dx.doi.org/10.1021/ac102261q
ISSN: 0003-2700
Appears in Collections:Closed Access (Mechanical, Electrical and Manufacturing Engineering)

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