Loughborough University
Leicestershire, UK
LE11 3TU
+44 (0)1509 263171
Loughborough University

Loughborough University Institutional Repository

Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/15857

Title: Numerical simulation of coupled cell motion and nutrient transport in NASA’s rotating bioreactor
Authors: Chao, Tzu-Chieh
Das, Diganta Bhusan
Issue Date: 2015
Publisher: © Elsevier
Citation: CHAO, T.-C. and DAS, D.B., 2015. Numerical simulation of coupled cell motion and nutrient transport in NASA’s rotating bioreactor. Chemical Engineering Journal, 259, pp. 961-971.
Abstract: Rotating bioreactor, such as the NASA bioreactor, which was designed by the National Aeronautics and Space Administration (NASA), USA, can be used to mimic micro-gravity conditions and simulate the effects of microgravity on cells growth. The cell growth in the bioreactor depends on the nutrient availability which in turn depends on the cell density and distribution within the bioreactor. In this work, we use a numerical model of suspended particle motion to simulate the cell motion and distribution in a specific variant of the NASA bioreactor, namely, the high aspect ratio vessel (HARV) bioreactor. The nutrient distribution in the bioreactor is simulated based on a convection-diffusion-reaction supplemented by laboratory experiments aimed at obtaining the required data. We present the modelling framework in this paper and discuss the most salient simulated results. For example, the simulation results show that the distributions of the cells in the bioreactor appear as concentric circles and that the cells density is higher in the middle of the HARV bioreactor. These cell distributions imply that they may accumulate in the middle of the bioreactor at sufficiently high cell density. The results also demonstrate that the concentration of nutrient is fairly uniform in the bioreactor but decreases slightly from the outer radius of the HARV bioreactor to the inner radius. This is possibly caused by the higher consumption of glucose due to the higher cell density in the middle of the radius. We expect that the modelling framework in this paper would help optimize the culture conditions for the cells in HARV bioreactors.
Description: NOTICE: this is the author’s version of a work that was accepted for publication in Chemical Engineering Journal. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Chemical Engineering Journal, 259, pp. 961-971, DOI: 10.1016/j.cej.2014.08.077
Version: Accepted for publication
DOI: 10.1016/j.cej.2014.08.077
URI: https://dspace.lboro.ac.uk/2134/15857
Publisher Link: http://dx.doi.org/10.1016/j.cej.2014.08.077
ISSN: 1385-8947
Appears in Collections:Published Articles (Chemical Engineering)

Files associated with this item:

File Description SizeFormat
Chao&Das_13Aug_Revised_Cleaned.pdfAccepted version1.67 MBAdobe PDFView/Open


SFX Query

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.