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|Title: ||Single cell tracking of gadolinium labeled CD4(+) T cells by laser ablation inductively coupled plasma mass spectrometry|
|Authors: ||Managh, Amy J.|
Edwards, Sheldon L.
Wood, Kathryn J.
Geissler, Edward K.
Hutchinson, James A.
Hutchinson, Robert W.
Reid, Helen J.
Sharp, Barry L.
|Issue Date: ||2013|
|Publisher: ||© American Chemical Society|
|Citation: ||MANAGH, A.J. ... et al, 2013. Single cell tracking of gadolinium labeled CD4(+) T cells by laser ablation inductively coupled plasma mass spectrometry. Analytical Chemistry, 85 (22), pp.10627-10634.|
|Abstract: ||Cellular therapy is emerging as a promising alternative to conventional immunosuppression in the fields of haematopoietic stem cell (HSC) transplantation, autoimmune disease and solid organ transplantation. Determining the persistence of cell-based therapies in vivo is crucial to understanding their regulatory function and requires the combination of an extremely sensitive detection technique and a stable, long-lifetime cell labelling agent. This paper reports the first application of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to perform single cell detection of T cell populations relevant to cellular immunotherapy. Purified human CD4+ T cells were labelled with commercially available Gd-based MRI contrast agents, Omniscan® and Dotarem®, which enabled passive loading of up to 108 Gd atoms per cell. In mixed preparations of labelled and unlabelled cells, LA-ICP-MS was capable of enumerating labelled cells at close to the predicted ratio. More importantly, LA-ICP-MS single cell analysis demonstrated that the cells retained sufficient label to remain detectable for up to 10 days post-labelling both in vitro and in vivo in an immunodeficient mouse model.|
|Description: ||This document is the Accepted Manuscript version of a Published Work that appeared in final form in
[Analytical Chemistry], copyright © American Chemical Society after peer review and technical editing by the publisher. The final edited and published work can be found at: http://dx.doi.org/10.1021/ac4022715|
|Sponsor: ||Support received from the ONE Study, a European
Commission Seventh Framework Program funded project
(EU FP7-HEALTH “The ONE Study”, Project reference
260687), and the British Heart Foundation PG 10/62/28504 is
|Version: ||Accepted for publication|
|Publisher Link: ||http://dx.doi.org/10.1021/ac4022715|
|Appears in Collections:||Published Articles (Chemistry)|
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