Loughborough University
Leicestershire, UK
LE11 3TU
+44 (0)1509 263171
Loughborough University

Loughborough University Institutional Repository

Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/16069

Title: Single cell tracking of gadolinium labeled CD4(+) T cells by laser ablation inductively coupled plasma mass spectrometry
Authors: Managh, Amy J.
Edwards, Sheldon L.
Bushell, Andrew
Wood, Kathryn J.
Geissler, Edward K.
Hutchinson, James A.
Hutchinson, Robert W.
Reid, Helen J.
Sharp, Barry L.
Issue Date: 2013
Publisher: © American Chemical Society
Citation: MANAGH, A.J. ... et al, 2013. Single cell tracking of gadolinium labeled CD4(+) T cells by laser ablation inductively coupled plasma mass spectrometry. Analytical Chemistry, 85 (22), pp.10627-10634.
Abstract: Cellular therapy is emerging as a promising alternative to conventional immunosuppression in the fields of haematopoietic stem cell (HSC) transplantation, autoimmune disease and solid organ transplantation. Determining the persistence of cell-based therapies in vivo is crucial to understanding their regulatory function and requires the combination of an extremely sensitive detection technique and a stable, long-lifetime cell labelling agent. This paper reports the first application of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to perform single cell detection of T cell populations relevant to cellular immunotherapy. Purified human CD4+ T cells were labelled with commercially available Gd-based MRI contrast agents, Omniscan® and Dotarem®, which enabled passive loading of up to 108 Gd atoms per cell. In mixed preparations of labelled and unlabelled cells, LA-ICP-MS was capable of enumerating labelled cells at close to the predicted ratio. More importantly, LA-ICP-MS single cell analysis demonstrated that the cells retained sufficient label to remain detectable for up to 10 days post-labelling both in vitro and in vivo in an immunodeficient mouse model.
Description: This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Analytical Chemistry], copyright © American Chemical Society after peer review and technical editing by the publisher. The final edited and published work can be found at: http://dx.doi.org/10.1021/ac4022715
Sponsor: Support received from the ONE Study, a European Commission Seventh Framework Program funded project (EU FP7-HEALTH “The ONE Study”, Project reference 260687), and the British Heart Foundation PG 10/62/28504 is gratefully acknowledged.
Version: Accepted for publication
DOI: 10.1021/ac4022715
URI: https://dspace.lboro.ac.uk/2134/16069
Publisher Link: http://dx.doi.org/10.1021/ac4022715
ISSN: 0003-2700
Appears in Collections:Published Articles (Chemistry)

Files associated with this item:

File Description SizeFormat
Gadolinium paper - final version.pdfAccepted version389.3 kBAdobe PDFView/Open


SFX Query

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.