Allogeneic non-adherent bone marrow cells facilitate hematopoietic recovery but do not lead to allogeneic engraftment..pdf (661.75 kB)
Allogeneic non-adherent bone marrow cells facilitate hematopoietic recovery but do not lead to allogeneic engraftment
journal contribution
posted on 2014-12-04, 11:50 authored by Stephan Fricke, Manuela Ackermann, Alexandra StolzingAlexandra Stolzing, Christoph Schimmelpfennig, Nadja Hilger, Jutta Jahns, Guido Hildebrandt, Frank Emmrich, Peter Ruschpler, Claudia Posel, Manja Kamprad, Ulrich SackBackground: Non adherent bone marrow derived cells (NA-BMCs) have recently been described to give rise to multiple mesenchymal phenotypes and have an impact in tissue regeneration. Therefore, the effects of murine bone marrow derived NA-BMCs were investigated with regard to engraftment capacities in allogeneic and syngeneic stem cell transplantation using transgenic, human CD4, murine CD4, HLA-DR3 mice. Methodology/Principal Findings: Bone marrow cells were harvested from C57Bl/6 and Balb/c wild-type mice, expanded to NA-BMCs for 4 days and characterized by flow cytometry before transplantation in lethally irradiated recipient mice. Chimerism was detected using flow cytometry for MHC-I (H-2D [b], H-2K[d]), mu/huCD4, and huHLA-DR3). Culturing of bone marrow cells in a dexamethasone containing DMEM medium induced expansion of non adherent cells expressing CD11b, CD45, and CD90. Analysis of the CD45 showed depletion of CD4, CD8, CD19, and CD117 cells. Expanded syngeneic and allogeneic NA-BMCs were transplanted into triple transgenic mice. Syngeneic NA-BMCs protected 83% of mice from death (n = 8, CD4 donor chimerism of 5.8±2.4% [day 40], P<.001). Allogeneic NA-BMCs preserved 62.5% (n = 8) of mice from death without detectable hematopoietic donor chimerism. Transplantation of syngeneic bone marrow cells preserved 100%, transplantation of allogeneic bone marrow cells 33% of mice from death. Conclusions/Significance: NA-BMCs triggered endogenous hematopoiesis and induced faster recovery compared to bone marrow controls. These findings may be of relevance in the refinement of strategies in the treatment of hematological malignancies. © 2009 Fricke et al.
Funding
This study was supported in part by research funding from German Federal Ministry for Education and Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
PLoS ONEVolume
4Issue
7Citation
FRICKE, S ... et al, 2009. Allogeneic non-adherent bone marrow cells facilitate hematopoietic recovery but do not lead to allogeneic engraftment. PLoS ONE, 4(7): e6157, 11pp.Publisher
Public Library Science (© 2009 Fricke et al)Version
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution 2.0 Unported (CC BY 2.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/2.0/Publication date
2009Notes
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.eISSN
1932-6203Publisher version
Language
- en