Thesis-2004-Barrett.pdf (12.1 MB)
Polymers in microfluidics
thesis
posted on 2015-01-20, 09:59 authored by Louise M. BarrettThere is great interest in miniaturized analytical systems for life science research, the
clinical environment, drug discovery, biotechnology, quality control, and environmental
monitoring and numerous articles have been written which predict the success of
microfluidic based systems. It was demonstrated m this work that a microfluidic flow
system could be quickly and easily manufactured in a research lab environment without
the need for clean room facilities. The microfluidic device was created using
polymethyhnethacrylate, a CO2 laser and an standard oven. The device was designed,
manufactured and ready for use within three hours. This work also investigated a
chemiluminescent system which was intended for use in protease assays in the
microfluidic device.
This work also focused on the use of photoinitiated polymer monoliths, with immobilized
tannic acid, as protein preconcentrators. The function of the monolithic devices was
demonstrated by pumping low concentration solutions of BSA BODIPY® FL through
the monolith. Both loading and elution were done using pressure. It was shown that
BSA could be concentrated on and successfully eluted from the monolith. The elution
volume for a 125 nI monolith was found to be 4 Ill. Therefore an injection of a 60 III
sample of 1 x 10-'\1 BSA BODIPY ® FL gave rise to a concentration factor of 15. The
pH optimum for the binding of BSA BODIPY ® FL was found to be pH 8.0 and the
loading capacity of the tannic acid monolith was found to be 0.6 mg_ml-1
History
School
- Science
Department
- Chemistry
Publisher
© Louise M. BarrettPublisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2004Notes
A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy of Loughborough University.EThOS Persistent ID
uk.bl.ethos.413588Language
- en