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Title: Single-cell tracking of therapeutic cells using Laser Ablation–Inductively Coupled Plasma–Mass Spectrometry
Authors: Managh, Amy J.
Keywords: Single cell analysis
Cell tracking
Laser ablation
ICP-MS
MRI contrast agents
Gold nanoparticles
Computational fluid dynamics
T-cells
M-regs
Tol-DC
Issue Date: 2014
Publisher: © Amy Joan Managh
Abstract: Cellular therapy is emerging as a clinically viable strategy in the field of solid organ transplantation, where it is expected to reduce the dependency on conventional immunosuppression. This has produced a demand for highly sensitive methods to monitor the persistence and tissue distribution of administered cells in vivo. However, tracking cells presents significant challenges. In many cases transplanted cells are autologous with the immune system of the transplant recipient, and hence are invisible to typical methods of detection. To enable their differentiation, the cells must be labelled with a suitable, non-toxic and long lifetime label, prior to their administration to patients. In addition, administered cells represent only a small fraction of the recipient’s endogenous cells, which necessitates the use of an extremely sensitive detection method. Laser ablation – inductively coupled plasma – mass spectrometry (LA-ICP-MS) is an exquisitely sensitive analytical technique, capable of imaging trace elements in complex samples, at high spatial resolution. [Continues.]
Description: A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy of Loughborough University.
Sponsor: European Commission 7th Framework Program
URI: https://dspace.lboro.ac.uk/2134/16723
Appears in Collections:PhD Theses (Chemistry)

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