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Title: Multiplexed, label-free detection of biomarkers using aptamers and Tunable Resistive Pulse Sensing (AptaTRPS).
Authors: Billinge, Emily R.
Platt, Mark
Keywords: Aptamers
Point of care
Tunable Resistive Pulse Sensing
Issue Date: 2015
Publisher: © Elsevier B.V
Citation: BILLINGE, E.R. and PLATT, M., 2015. Multiplexed, label-free detection of biomarkers using aptamers and Tunable Resistive Pulse Sensing (AptaTRPS). Biosensors and Bioelectronics, 68, pp. 741 - 748.
Abstract: Diagnostics that are capable of detecting multiple biomarkers are improving the accuracy and efficiency of bioassays. In previous work we have demonstrated the potential of an aptamer-based sensor (aptasensor) utilising Tunable Resistive Pulse Sensing (TRPS). Here, we have advanced the technique identifying key experimental designs for potential POC assays. The assay utilised superparamagnetic beads, and using TRPS monitored their translocations through a pore. If the surfaces of the beads are modified with an aptamer, the frequency of beads (translocations/min) through the pore can be related to the concentration of specific proteins in the solution. Herein, we have demonstrated the successful use of TRPS to observe the binding of two proteins, to their specific aptamers simultaneously. We describe a series of experiments illustrating key factors which we believe are integral to bead-based assays and demonstrate a general method for a multiplexed assay. In summary, we have explored the effects of beads size, concentration, potential bias, pH and aptamer affinity to enhance the sensitivity and practically of a TRPS aptasensor. The method utilises the fact the binding of the aptamer to the protein results in a change in charge density on the bead surface, the isoelectric point of the protein then dominates the mobility of the beads, creating a multiplexed assay termed AptaTRPS. By alteration of the applied potential to the instrument it is possible to produce a positive signal in a simple multiplexed assay.
Description: NOTICE: this is the author’s version of a work that was accepted for publication in Biosensors and Bioelectronics. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biosensors and Bioelectronics, vol 68, June 2015, DOI: 10.1016/j.bios.2015.02.011
Sponsor: The authors thank the Centre for Analytical Science at Loughborough University. The work was supported by the European Commission for Research (PCIG11-GA-2012-321836 Nano4Bio) and Loughborough University Chemistry Department (Start-up fund). The research was supported by the National Institute for Health Research (NIHR) Diet, Lifestyle & Physical Activity Biomedical Research Unit based at University Hospitals of Leicester and Loughborough University.
Version: Accepted for publication
DOI: 10.1016/j.bios.2015.02.011
URI: https://dspace.lboro.ac.uk/2134/16838
Publisher Link: http://dx.doi.org/10.1016/j.bios.2015.02.011
Appears in Collections:Published Articles (Chemistry)

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