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Stressed stem cells: temperature response in aged mesenchymal stem cells
journal contribution
posted on 2015-03-04, 11:05 authored by Alexandra StolzingAlexandra Stolzing, Sebastian Sethe, Andrew ScuttMesenchymal stem cells (MSCs) derived from young (6 week) and aged (56 week) Wistar rats were cultured at standard (37°C) and reduced (32°C) temperature and compared for age markers and stress levels, (ROS, NO, TBARS, carbonyls, lipofuscin, SOD, GPx, apoptosis, proteasome activity) and heat shock proteins (HSP27, -60, -70, -90). Aged MSCs display many of the stress markers associated with aging in other cell types, but results vary across marker categories and are temperature dependant. In young MSCs, culturing at reduced temperature had a generally beneficial effect: the anti-apoptotic heat shock proteins HSP 27, HSP70, and HSP90 were up-regulated; pro-apoptotic HSP60 was downregulated; SOD, GPx increased; and levels in ROS, NO, TBARS, carbonyl, and lipofuscin were diminished. Apoptosis was reduced, but also proteasome activity. In contrast, in aged MSCs, culturing at reduced temperature generally produced no 'beneficial' changes in these parameters, and can even have detrimental effects. Implications for tissue engineering and for stem cell gerontology are discussed. The results suggest that a 'hormesis' theory of stress response can be extended to MSCs, but that cooling cultivation temperature stress produces positive effects in young cells only. © Mary Ann Liebert, Inc.
Funding
BBSRC
History
School
- Mechanical, Electrical and Manufacturing Engineering
Published in
Stem Cells and DevelopmentVolume
15Issue
4Pages
478 - 487Citation
STOLZING, A., SETHE, S. and SCUTT, A.M., 2006. Stressed stem cells: temperature response in aged mesenchymal stem cells. Stem Cells and Development, 15 (4), pp. 478 - 487.Publisher
© Mary Ann Liebert, Inc.Version
- VoR (Version of Record)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2006Notes
Closed access. Final publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/10.1089/scd.2006.15.478.ISSN
1547-3287Publisher version
Language
- en