Loughborough University
Browse

File(s) under permanent embargo

Reason: This item is currently closed access.

Neuronal apoptotic bodies: phagocytosis and degradation by primary microglial cells.

journal contribution
posted on 2015-03-05, 15:26 authored by Alexandra StolzingAlexandra Stolzing, Tilman Grune
Neuronal loss via apoptosis is a key element in numerous neurodegenerative diseases. To avoid accumulation of apoptotic material, the remains of apoptotic cells should be degraded. It was suggested that microglial cells are phagocytosing and degrading apoptotic material. There is only limited information available concerning the fate of the remains of apoptotic neurons. In this study, we investigated the ability of microglial cells to take up and degrade neuronal apoptotic material. We isolated primary microglial cells and used apoptotic bodies of apoptotic neuron-like PC12 cells as a substrate. The apoptotic material was taken up and degraded within the microglial cells. The uptake is clearly activation dependent. We were able to demonstrate that the CD36 scavenger receptor is involved in the uptake of the apoptotic material via competition studies, antibody blockage, and use of a CD36 mutant rat strain. Blockage of other uptake mechanisms was also able to inhibit the uptake to some extent. Furthermore, we were able to demonstrate the role of the microglial lysosomal and proteasomal pathways in the degradation of proteins originating from apoptotic bodies.

Funding

T.G. was sponsored by the DFG (DFG 1240/9-1 and SFB 507/A7).

History

School

  • Mechanical, Electrical and Manufacturing Engineering

Published in

The FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Volume

18

Issue

6

Pages

743 - 745

Citation

STOLZING, A. and GRUNE, T., 2004. Neuronal apoptotic bodies: phagocytosis and degradation by primary microglial cells. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 18 (6), pp. 743 - 745.

Publisher

© Federation of American Society of Experimental Biology (FASEB)

Version

  • VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2004

Notes

This article is closed access.

eISSN

1530-6860

Language

  • en