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Title: Neuronal apoptotic bodies: phagocytosis and degradation by primary microglial cells.
Authors: Stolzing, Alexandra
Grune, Tilman
Keywords: Proteasome
Lysosome
Scavenger receptor
Microglia
Protein oxidation
Issue Date: 2004
Publisher: © Federation of American Society of Experimental Biology (FASEB)
Citation: STOLZING, A. and GRUNE, T., 2004. Neuronal apoptotic bodies: phagocytosis and degradation by primary microglial cells. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 18 (6), pp. 743 - 745.
Abstract: Neuronal loss via apoptosis is a key element in numerous neurodegenerative diseases. To avoid accumulation of apoptotic material, the remains of apoptotic cells should be degraded. It was suggested that microglial cells are phagocytosing and degrading apoptotic material. There is only limited information available concerning the fate of the remains of apoptotic neurons. In this study, we investigated the ability of microglial cells to take up and degrade neuronal apoptotic material. We isolated primary microglial cells and used apoptotic bodies of apoptotic neuron-like PC12 cells as a substrate. The apoptotic material was taken up and degraded within the microglial cells. The uptake is clearly activation dependent. We were able to demonstrate that the CD36 scavenger receptor is involved in the uptake of the apoptotic material via competition studies, antibody blockage, and use of a CD36 mutant rat strain. Blockage of other uptake mechanisms was also able to inhibit the uptake to some extent. Furthermore, we were able to demonstrate the role of the microglial lysosomal and proteasomal pathways in the degradation of proteins originating from apoptotic bodies.
Description: This article is closed access.
Sponsor: T.G. was sponsored by the DFG (DFG 1240/9-1 and SFB 507/A7).
Version: Published
DOI: 10.1096/fj.03-0374fje.
URI: https://dspace.lboro.ac.uk/2134/16898
Publisher Link: http://dx.doi.org/10.1096/fj.03-0374fje
Appears in Collections:Closed Access (Mechanical, Electrical and Manufacturing Engineering)

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