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Title: Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells
Authors: Ernst, Andrea
Stolzing, Alexandra
Sandig, Grit
Grune, Tilman
Keywords: Antioxidants
Free radicals
Oligodendrocytes
OLN93 cells
Protein oxidation
Issue Date: 2004
Publisher: © Elsevier Inc
Citation: ERNST, A. ... et al., 2004. Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells. Archives of Biochemistry and Biophysics, 421 (1), pp. 54 - 60.
Abstract: Oxidative stress is supposed to play an important role in demyelinating diseases. Oligodendrocytes are the myelin-forming cells in the brain and are highly susceptible to oxidative stress due to their low antioxidative defense systems and high metabolic rate. In the present work, we tested the response of the oligodendrocyte cell line OLN 93 to oxidative stress. OLN 93 cell cultures are characterized by a loss of cell viability after oxidation. This loss of cell viability is accompanied by an increase in protein oxidation and consequently an elevated overall proteolysis. To minimize the oxidative damage, we tested the effects of the antioxidants α-lipoic acid and coenzyme Q10. Both compounds were able to elevate cell viability and to decrease intracellular protein turnover and oxidant induced protein oxidation. Therefore, we concluded that the excessive oxidative damage of oligodendrocytes and their protein pool can be prevented by the usage of antioxidants.
Description: Closed access
Sponsor: Deutsche Forschungsgemeinschaft and the Charite Research Funds
Version: Published
DOI: 10.1016/j.abb.2003.10.008
URI: https://dspace.lboro.ac.uk/2134/16902
Publisher Link: http://dx.doi.org/10.1016/j.abb.2003.10.008
ISSN: 0003-9861
Appears in Collections:Closed Access (Mechanical, Electrical and Manufacturing Engineering)

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