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Title: Degradation of oxidized extracellular proteins by microglia
Authors: Stolzing, Alexandra
Wengner, Antje
Grune, Tilman
Keywords: Microglia
Protein degradation
Protein oxidation
Issue Date: 2002
Publisher: © Elsevier Science
Citation: Stolzing, A., WENGNER, A. and GRUNE, T., 2002. Degradation of oxidized extracellular proteins by microglia. Archives of Biochemistry and Biophysics, 400 (2), pp. 171 - 179.
Abstract: In living organisms a permanent oxidation of protein oxidation occurs. The degradation of intracellular oxidized proteins is intensively studied, but knowledge about the fate of oxidatively modified extracellular proteins is still limited. We studied the fate of exogenously added oxidized proteins in microglial cells. Both primary microglial cells and RAW cells are able to remove added oxidized laminin and myelin basic protein from the extracellular environment. Moderately oxidized proteins are degraded most efficiently, whereas strongly oxidized proteins are taken up by the microglial cells without an efficient degradation. Activation of microglial cells enhances the selective recognition and degradation of moderately oxidized protein substrates by proteases. Inhibitor studies also revealed an involvement of the lysosomal and the proteasomal system in the degradation of extracellular proteins. These studies let us conclude that microglial cells are able to remove oxidized proteins from the extracellular environment in the brain. © 2002 Elsevier Science (USA). All rights reserved.
Description: This article is closed access.
Sponsor: This study was supported by the Deutsche Forschungsgemeinschaft (DFG Gr 1240/9-1).
Version: Published
DOI: 10.1016/S0003-9861(02)00003-6
URI: https://dspace.lboro.ac.uk/2134/16917
Publisher Link: http://dx.doi.org/10.1016/S0003-9861(02)00003-6
ISSN: 0003-9861
Appears in Collections:Closed Access (Mechanical, Electrical and Manufacturing Engineering)

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