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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/16947

Title: Cryopreservation of human umbilical cord-derived mesenchymal stem cells in complex sugar based cryoprotective solutions
Authors: Naaldijk, Yahaira
Federova, Viktoriya
Stolzing, Alexandra
Keywords: Mesenchymal stem cells
Issue Date: 2013
Publisher: Bioinfo Publications / © The Authors
Citation: NAALDIJK, Y., FEDEROVA, V. and STOLZING, A., 2013. Cryopreservation of human umbilical cord-derived mesenchymal stem cells in complex sugar based cryoprotective solutions. Journal of Biotechnology Letters, 4 (2), pp. 95 - 102.
Abstract: Mesenchymal stem cells (MSCs) are able to differentiate in vivo and in vitro giving rise to different cell types including osteoblasts, adipocytes, chondrocytes and neuronal cells, providing a valuable source for treatment of degenerative and age-associated diseases. Im-provement of protocols and procedures for human MSCs cryopreservation will contribute significantly to the development of cell replacement therapies. We developed an alternative cryopreservation solutions for stem cell cryopreservation. Most cryoprotectants need to be removed from the cells by washing after thawing, a procedure that can lead to a loss of precious stem cells. Additionally, the procedure is time and cost-consuming. In our study we used a combination of transfusable and non-toxic substances such as hydroxyethylstarch, sorbitol and dextran replacing DMSO and FCS. We found that a cryosolution containing 5% HES, 0.3M sorbitol and 5% dextran provide successful protection for human umbilical cord derived mesenchymal stem cells. These MSC retain a high viability and show multilineage differentiation.
Description: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Sponsor: The work presented on this paper was made possible by fundings from SerumWerk GmbH, the Sächsische-Anhalt Aufbaubank and the German Federal Ministry of Education and Research (BMBF 1315883).
Version: Published
URI: https://dspace.lboro.ac.uk/2134/16947
Publisher Link: http://bioinfopublication.org/journal.php?opt=azjou&jouid=BPJ0000250
ISSN: 0976-7045
Appears in Collections:Published Articles (Mechanical, Electrical and Manufacturing Engineering)

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