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|Title: ||Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device|
|Authors: ||Barbosa, Ana I.|
Edwards, Alexander D.
Reis, Nuno M.
Point of care diagnostics
|Issue Date: ||2015|
|Publisher: ||Elsevier B.V. (© 2015 The Authors)|
|Citation: ||BARBOSA, A. ... et al, 2015. Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device. Biosensors and Bioelectronics, 70, pp.5-14.|
|Abstract: ||We present a new, power-free and flexible detection system named MCFphone for portable colorimetric and fluorescence quantitative sandwich immunoassay detection of prostate specific antigen (PSA). The MCFphone is composed by a smartphone integrated with a magnifying lens, a simple light source and a miniaturised immunoassay platform, the Microcapillary Film (MCF). The excellent transparency and flat geometry of fluoropolymer MCF allowed quantitation of PSA in the range 0.9 to 60. ng/ml with<7% precision in 13. min using enzymatic amplification and a chromogenic substrate. The lower limit of detection was further improved from 0.4 to 0.08. ng/ml in whole blood samples with the use of a fluorescence substrate. The MCFphone has shown capable of performing rapid (13 to 22. min total assay time) colorimetric quantitative and highly sensitive fluorescence tests with good %Recovery, which represents a major step in the integration of a new generation of inexpensive and portable microfluidic devices with commercial immunoassay reagents and off-the-shelf smartphone technology.|
|Description: ||This article was published as Open Access by Elsevier B.V. under the CC BY license(http://creativecommons.org/licenses/by/4.0/).|
|Sponsor: ||This work was co-funded by Capillary Film Technology Ltd, Loughborough University and EPSRC [grant no. EP/L013983/1].|
|Publisher Link: ||http://dx.doi.org/10.1016/j.bios.2015.03.006|
|Appears in Collections:||Published Articles (Chemical Engineering)|
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