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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/17336

Title: Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device
Authors: Barbosa, Ana I.
Gehlot, Poonam
Sidapra, Kalpita
Edwards, Alexander D.
Reis, Nuno M.
Keywords: Colorimetric
Miniaturised ELISA
Point of care diagnostics
Smartphone readout
Issue Date: 2015
Publisher: Elsevier B.V. (© 2015 The Authors)
Citation: BARBOSA, A. ... et al, 2015. Portable smartphone quantitation of prostate specific antigen (PSA) in a fluoropolymer microfluidic device. Biosensors and Bioelectronics, 70, pp.5-14.
Abstract: We present a new, power-free and flexible detection system named MCFphone for portable colorimetric and fluorescence quantitative sandwich immunoassay detection of prostate specific antigen (PSA). The MCFphone is composed by a smartphone integrated with a magnifying lens, a simple light source and a miniaturised immunoassay platform, the Microcapillary Film (MCF). The excellent transparency and flat geometry of fluoropolymer MCF allowed quantitation of PSA in the range 0.9 to 60. ng/ml with<7% precision in 13. min using enzymatic amplification and a chromogenic substrate. The lower limit of detection was further improved from 0.4 to 0.08. ng/ml in whole blood samples with the use of a fluorescence substrate. The MCFphone has shown capable of performing rapid (13 to 22. min total assay time) colorimetric quantitative and highly sensitive fluorescence tests with good %Recovery, which represents a major step in the integration of a new generation of inexpensive and portable microfluidic devices with commercial immunoassay reagents and off-the-shelf smartphone technology.
Description: This article was published as Open Access by Elsevier B.V. under the CC BY license(http://creativecommons.org/licenses/by/4.0/).
Sponsor: This work was co-funded by Capillary Film Technology Ltd, Loughborough University and EPSRC [grant no. EP/L013983/1].
Version: Published
DOI: 10.1016/j.bios.2015.03.006
URI: https://dspace.lboro.ac.uk/2134/17336
Publisher Link: http://dx.doi.org/10.1016/j.bios.2015.03.006
ISSN: 0956-5663
Appears in Collections:Published Articles (Chemical Engineering)

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